Oshikawa Katsuhisa, Yanagisawa Ken, Ohno Shoji, Tominaga Shin-Ichi, Sugiyama Yukihiko
Division of Pulmonary Medicine, Department of Medicine, Jichi Medical School, Minamikawachi, Tochigi, Japan.
Am J Respir Crit Care Med. 2002 Apr 1;165(7):1005-9. doi: 10.1164/ajrccm.165.7.2105109.
The objective of this study was to test the hypothesis that accumulated helper T lymphocytes in malignant pleural effusions may shift to T-helper type 2 (Th2) and produce soluble ST2 protein. We took samples of serum and pleural effusions (p-) from patients with carcinomatous pleurisy (CA, n = 17), tuberculous pleurisy (TB, n = 8), and congestive heart failure (HF, n = 5) and compared the concentration of cytokines or ST2. Ex vivo production of interleukin (IL)-4 and IL-10, though not that of interferon (IFN)-gamma or IL-12, from CD4+ T cells isolated from pleural effusions was higher in the CA group than in the TB or HF group. The p-ST2 concentrations were significantly higher in the CA group than in the TB or HF group, positively correlated with the percentage of pleural effusion CD4+ T cells (r = 0.432, p = 0.016) and inversely correlated with p-IFN-gamma concentrations (r = -0.423, p = 0.019). Furthermore, mRNA expression of ST2 in CD4+ T cells isolated from group CA was upregulated, compared with that in those isolated from the TB group. These results suggest that CD4+ T cells in CA shift to Th2, which can produce soluble ST2 protein, resulting in increased concentrations of p-ST2 in malignant pleural effusion.
恶性胸腔积液中积累的辅助性T淋巴细胞可能转变为2型辅助性T细胞（Th2）并产生可溶性ST2蛋白。我们采集了癌性胸膜炎（CA，n = 17）、结核性胸膜炎（TB，n = 8）和充血性心力衰竭（HF，n = 5）患者的血清和胸腔积液样本（p-），并比较了细胞因子或ST2的浓度。与TB组或HF组相比，CA组从胸腔积液中分离出的CD4 + T细胞产生白细胞介素（IL）-4和IL-10的体外产量较高，而干扰素（IFN）-γ或IL-12的产量则不然。CA组的p-ST2浓度显著高于TB组或HF组，与胸腔积液CD4 + T细胞百分比呈正相关（r = 0.432，p = 0.016），与p-IFN-γ浓度呈负相关（r = -0.423，p = 0.019）。此外，与从TB组分离出的CD4 + T细胞相比，从CA组分离出的CD4 + T细胞中ST2的mRNA表达上调。这些结果表明，CA中的CD4 + T细胞转变为Th2，后者可产生可溶性ST2蛋白，导致恶性胸腔积液中p-ST2浓度升高。
