Oertel J, Kingreen D, Busemann C, Stein H, Dörken B
Haematology-Oncology Department, Charité, Humboldt University Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
J Cancer Res Clin Oncol. 2002 Apr;128(4):182-8. doi: 10.1007/s00432-001-0311-4. Epub 2002 Feb 1.
This study analysed the morphologic differences between leukaemic mantle cell lymphoma, follicular lymphoma, nodal marginal zone lymphoma, and diffuse large B-cell lymphoma in peripheral blood. Additionally, we investigated the role of cyclin D1 expression in B-lymphoproliferative disorders.
The morphologic analysis of the leukaemic cells was performed on cytocentrifuge preparations after separation of mononuclear cells from peripheral blood using a Ficoll-Hypaque density gradient. Cyclin D1 protein expression was studied with the catalyzed signal amplification system. The expression of other markers (CD5, CD23, light chain immunoglobulins) was analysed by the APAAP method.
We describe in detail the morphology of the lymphoma cells in eight patients with mantle cell lymphoma, six patients with follicular lymphoma, 11 patients with nodal marginal zone lymphoma, and seven patients with diffuse large B-cell lymphoma. The morphological distinction between these lymphoma cells is a challenge for the haematologist. The investigation of cytocentrifuge preparations of mononuclear cells allows the detection of lymphoma cells also in cases with nondiagnostic white cell differential. Additionally, the immunotype (light chain restriction, CD5, CD23, and cyclin D1) of 108 patients with leukaemic B-lymphoproliferative disorders was studied. Diffuse nuclear expression of cyclin D1 protein (>20%) was specific for mantle cell lymphoma. However, only 6/8 patients showed cyclin-D1 positivity.
The morphologic analysis of lymphoma cells in cytocentrifuge preparations of mononuclear leukocytes in combination with immunocytochemical investigation allows the detection of mantle cells, centrocytes of follicular lymphoma, marginal zone cells, and cells of the diffuse large B-cell lymphoma in peripheral blood. The positivity of cyclin D1 protein improves the differentiation of mantle cells from other lymphoma cells.
本研究分析了外周血中白血病性套细胞淋巴瘤、滤泡性淋巴瘤、结内边缘区淋巴瘤和弥漫性大B细胞淋巴瘤之间的形态学差异。此外，我们研究了细胞周期蛋白D1表达在B淋巴细胞增殖性疾病中的作用。
使用Ficoll-Hypaque密度梯度从外周血中分离单核细胞后，在细胞离心涂片上进行白血病细胞的形态学分析。采用催化信号放大系统研究细胞周期蛋白D1蛋白表达。通过碱性磷酸酶抗碱性磷酸酶（APAAP）法分析其他标志物（CD5、CD23、轻链免疫球蛋白）的表达。
我们详细描述了8例套细胞淋巴瘤、6例滤泡性淋巴瘤、11例结内边缘区淋巴瘤和7例弥漫性大B细胞淋巴瘤患者淋巴瘤细胞的形态。这些淋巴瘤细胞之间的形态学区分对血液学家来说是一项挑战。对单核细胞的细胞离心涂片进行研究，即使在白细胞分类未明确诊断的情况下也能检测到淋巴瘤细胞。此外，我们研究了108例白血病性B淋巴细胞增殖性疾病患者的免疫表型（轻链限制、CD5、CD23和细胞周期蛋白D1）。细胞周期蛋白D1蛋白弥漫性核表达（>20%）对套细胞淋巴瘤具有特异性。然而，仅6/8例患者显示细胞周期蛋白D1阳性。
对单核白细胞的细胞离心涂片进行淋巴瘤细胞形态学分析并结合免疫细胞化学研究，能够在外周血中检测到套细胞、滤泡性淋巴瘤的中心细胞、边缘区细胞和弥漫性大B细胞淋巴瘤细胞。细胞周期蛋白D1蛋白阳性有助于套细胞与其他淋巴瘤细胞的鉴别。
