Kaczmarczyk R, Słodziński H, Trojanowski T, Polz-Dacewicz M
Katedry i Kliniki Neurochirurgii i Neurochirurgii Dzieciecej AM w Lublinie.
Neurol Neurochir Pol. 2001;35 Suppl 5:30-3.
There are conflicting reports in connection with the association of the p53 tumour suppressor gene mutation with the clinical and histopathological progression of gliomas. Glia-derived neoplasms frequently show mutational changes in the p53 gene which result in enhancement of tumorigenesis. The aim of the paper was an assessment of the frequency of mutations in the exon 8 of this gene. The specimens from 14 patients operated for glial tumors were investigated by polymerase chain reaction-assisted--single strand conformation polymorphism (PCR-SSCP). We found aberrant bands in 64.3% of specimens. The percentage of mutations was similar in patients with benign and malignant tumours. There was no correlation between the alteration of the gene and intensity of necrosis in histological examination in patients with glioblastoma. Changes in activity of the p53 gene were more frequent in younger patients and in males when compared to women.
关于p53肿瘤抑制基因突变与神经胶质瘤临床及组织病理学进展之间的关联，存在相互矛盾的报道。源自神经胶质细胞的肿瘤常常显示出p53基因的突变变化，这会导致肿瘤发生增强。本文的目的是评估该基因第8外显子的突变频率。对14例接受神经胶质瘤手术患者的标本进行了聚合酶链反应辅助单链构象多态性分析（PCR-SSCP）。我们在64.3%的标本中发现了异常条带。良性和恶性肿瘤患者的突变百分比相似。在胶质母细胞瘤患者的组织学检查中，基因改变与坏死强度之间没有相关性。与女性相比，p53基因活性变化在年轻患者和男性中更为频繁。
