Yokoyama Kenji, Koide Satoshi, Kayanuma Yoshihiro
School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa.
Anal Bioanal Chem. 2002 Jan;372(2):248-53. doi: 10.1007/s00216-001-1203-9. Epub 2001 Dec 18.
A cyclic voltammetric simulation that can be applied to an electrochemically mediated enzyme reaction involving any substrate and mediator concentration was developed. Concentration polarization of the substrate in the vicinity of an electrode was considered as well as mediator concentration. Reversible electrochemical reaction with one electron followed by an enzyme reaction with two electrons was modeled. The differential equations for the mediator and substrate were solved using digital simulation techniques. The calculated cyclic voltammograms showed prepeaks when there was a low substrate concentration, high mediator concentration, and high enzyme activity. The prepeak was experimentally observed in the case of an enzyme electrode co-immobilized with a redox polymer. The enzyme electrode loaded at high redox polymer and high enzyme content showed a prepeak at low substrate concentration in the cyclic voltammogram.
