变形链球菌突变体产生高水平的葡聚糖蔗糖酶。
Production of elevated levels of dextransucrase by a mutant of Streptococcus mutans.
作者信息
Schachtele C F, Germaine G R, Harlander S K
出版信息
Infect Immun. 1975 Oct;12(4):934-7. doi: 10.1128/iai.12.4.934-937.1975.
Abstract
A mutant (S19) of Sreptococcus mutans strain 6715 which produces elevated levels of dextransucrase (EC 2.4.1.5) was isolated. Soluble enzyme in culture supernatant solutions from S19 polymerized the glucosyl moiety of sucrose into alcohol-insoluble and water-insoluble glucans at a rate three to six times greater than that of the parent strain. Washed-cell suspensions of S19 also contained increased amounts of cell-associated enzyme. Adherence of S19 to glass in the presence of sucrose occurred at twice the rate of strain 6715. The Km values for sucrose and primer dextran were similar for the mutant and parent enzymes. Mutant S19 should facilitate studies on the mechanism of adherence of S. Mutans and the control of dextransucrase production by this bacterium.
摘要
分离出变形链球菌6715菌株的一个突变体（S19），其葡聚糖蔗糖酶（EC 2.4.1.5）水平升高。来自S19的培养上清液中的可溶性酶将蔗糖的葡萄糖基部分聚合成醇不溶性和水不溶性葡聚糖的速率比亲本菌株快三到六倍。S19的洗涤细胞悬液中也含有更多的细胞相关酶。在蔗糖存在下，S19对玻璃的粘附速率是6715菌株的两倍。突变体和亲本酶对蔗糖和引物葡聚糖的米氏常数相似。突变体S19应有助于研究变形链球菌的粘附机制以及该细菌对葡聚糖蔗糖酶产生的控制。
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