变形链球菌葡聚糖蔗糖酶：在化学限定培养基中生长后解离酶的可得性
Streptococcus mutans dextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium.
作者信息
Schachtele C F, Harlander S K, Germaine G R
出版信息
Infect Immun. 1976 May;13(5):1522-4. doi: 10.1128/iai.13.5.1522-1524.1976.
Abstract
The soluble dextransucrase (EC 2.4.1.5) activity produced by Streptococcus mutans strain 6715 during growth on a chemically defined synthetic medium (FMS) was compared to enzyme from glucose broth cultures (TSB). Growth on the two media was similar. The specific activity of ammonium sulfate-precipitated FMC enzyme was 17 times greater than similar TSB enzyme preparations. The FMC enzyme was stimulated 11-fold, whereas the TSB enzyme was stimulated 1.2-fold by the addition of exogenous primer dextran. In contrast to the TSB enzyme, the FMC activity could be disaggregated to a low-molecular-weight form by 1 M salt. Thus, low-molecular-weight S. mutans dextransucrase activity free of contaminating primer glucan may be readily obtained after growth of the bacterium in a chemically defined sucrose-free medium.
摘要
比较了变形链球菌6715菌株在化学限定合成培养基（FMS）上生长期间产生的可溶性葡聚糖蔗糖酶（EC 2.4.1.5）活性与葡萄糖肉汤培养物（TSB）中的酶活性。在两种培养基上的生长情况相似。硫酸铵沉淀的FMC酶的比活性比相似的TSB酶制剂高17倍。添加外源引物葡聚糖后，FMC酶的活性提高了11倍，而TSB酶的活性提高了1.2倍。与TSB酶不同，FMC活性可以通过1 M盐解离成低分子量形式。因此，在无蔗糖的化学限定培养基中培养细菌后，可以很容易地获得不含污染引物葡聚糖的低分子量变形链球菌葡聚糖蔗糖酶活性。
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