Sun H, Gong Y, Wang Q, Chen J
Institute of Transfusion, Beijing 100850, China.
Wei Sheng Yan Jiu. 1999 Mar 30;28(2):95-6.
The malignant transformation of HPV-18 immortalized human bronchial epithelial cell(BEP2D) induced by tobacco-specific nitrosamine NNK and its biological characteristics were investigated. BEP2D cells treated with NNK at the concentration of 500 micrograms/ml for 24 hours could be subcultured continuously in vitro. The 15th passage cells in soft agar (0.038%) increased 11 fold compared with that of control cells (0.0033%). The 25th passage cells could grow into tumor in nude mice. The tumor was a squamous cell carcinoma in morphology confirmed by histopathological examination. The results indicated that the malignant transformation induced by NNK was a sequential process. This culture system provides a potential tool for the study of cell and molecular mechanism in the multistage carcinogenesis of human bronchial epithelial cells.
研究了烟草特异性亚硝胺NNK诱导人乳头瘤病毒18型永生化人支气管上皮细胞（BEP2D）的恶性转化及其生物学特性。用浓度为500微克/毫升的NNK处理BEP2D细胞24小时后，细胞能够在体外连续传代培养。软琼脂（0.038%）中第15代细胞相较于对照细胞（0.0033%）增加了11倍。第25代细胞能够在裸鼠体内长成肿瘤。经组织病理学检查证实，该肿瘤在形态上为鳞状细胞癌。结果表明，NNK诱导的恶性转化是一个渐进的过程。该培养系统为研究人支气管上皮细胞多阶段致癌过程中的细胞和分子机制提供了一个潜在工具。
