Liu Yang, Pan Jiaqi, Wang Shujie, Li Xiurong, Huang Yining
Department of Hematology, Peking Union Medical College Hospital, Beijing 100730, China.
Chin Med J (Engl). 2002 Feb;115(2):214-6.
To investigate the relationship between the beta-fibrinogen gene -455A/G polymorphism and plasma fibrinogen level and to determine the influence of the mutation on ischemic stroke.
Ninety-one patients (63.5 +/- 10.1 years) with ischemic stroke and 74 elderly control subjects (60.6 +/- 10.8 years) without any thromboembolic events and 98 healthy blood donators as young control (37.5 +/- 13.3 years) were enrolled in this trial. The beta-fibrinogen gene -455A/G polymorphism was analyzed for all subjects by PCR-RFLP with the restrictive enzyme Hae III, while plasma fibrinogen levels were obtained from the prothrombin time (PT) assay. For statistical analysis, the parameters were compared between any two different groups by the unpaired Student's t test and the Chi-square test. Before analysis, log transformations for concentrations of fibrinogen were carried out.
H2 allele frequency was higher in male ischemic stroke patients than in the elderly control (22.7% vs 7.1%, chi(2) = 5.56, P < 0.02). There was no significant difference between the female groups. In those patients without any thromboembolic events (both elderly and young control groups), the frequency of H2 decreased with age (< or = 40, 21.3%; 41 - 59, 15.4%; and > or = 60, 10.2%). In the male elderly and young control groups, the level of plasma fibrinogen was lower in the H1H1 genotype (287 +/- 96 mg/dl and 234 +/- 58 mg/dl) than in H1H2 and H2H2 (331 +/- 44 mg/dl and 307 +/- 55 mg/dl; t = 2.53 and 9.67, P < 0.05). In the female elderly groups, this tendency was not found.
Plasma fibrinogen expression is affected by the beta-fibrinogen gene -455A/G polymorphism, and the H2 allele may be a risk factor for ischemic stroke in Chinese males.
研究β-纤维蛋白原基因-455A/G多态性与血浆纤维蛋白原水平之间的关系，并确定该突变对缺血性脑卒中的影响。
本试验纳入91例缺血性脑卒中患者（63.5±10.1岁）、74例无任何血栓栓塞事件的老年对照者（60.6±10.8岁）和98例健康献血者作为年轻对照（37.5±13.3岁）。采用限制性内切酶Hae III通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析所有受试者的β-纤维蛋白原基因-455A/G多态性，同时通过凝血酶原时间（PT）测定获得血浆纤维蛋白原水平。进行统计学分析时，采用非配对学生t检验和卡方检验比较任意两组不同参数。分析前，对纤维蛋白原浓度进行对数转换。
男性缺血性脑卒中患者的H2等位基因频率高于老年对照组（22.7%对7.1%，χ² = 5.56，P < 0.02）。女性组之间无显著差异。在无任何血栓栓塞事件的患者中（老年和年轻对照组），H2频率随年龄降低（≤40岁，21.3%；41 - 59岁，15.4%；≥60岁，10.2%）。在老年男性和年轻男性对照组中，H1H1基因型的血浆纤维蛋白原水平（287±96mg/dl和234±58mg/dl）低于H1H2和H2H2基因型（331±44mg/dl和307±55mg/dl；t = 2.53和9.67，P < 0.05）。在老年女性组中，未发现这种趋势。
血浆纤维蛋白原表达受β-纤维蛋白原基因-455A/G多态性影响，H2等位基因可能是中国男性缺血性脑卒中的危险因素。
