成年大鼠脊髓损伤后室管膜细胞的巢蛋白表达与增殖
Nestin expression and proliferation of ependymal cells in adult rat spinal cord after injury.
作者信息
Liu Kui, Wang Zhongcheng, Wang Hongyun, Zhang Yazhuo
机构信息
Beijing Neurosurgical Institute, Beijing 100050, China.
出版信息
Chin Med J (Engl). 2002 Mar;115(3):339-41.
OBJECTIVE
To determine cell proliferation and nestin expression in the ependyma of adult rat spinal cord after injury.
METHODS
Rat spinal cord injury models were established by aneurysm clip compression, and nestin expression and proliferation of ependymal cells at different times were shown with pathological and immuno-histochemical staining.
RESULTS
Ependymal cells adjacent to the injured site demonstrated a dramatic increase in nestin expression 24 hours after compression. Proliferating cell nuclear antigen was positive, and significant proliferation was observed after 7 days. Nestin expression was down regulated as time went by.
CONCLUSION
Normally quiescent mature ependymal cells appear to revert to an embryonic state in response to spinal cord injury.
目的
确定成年大鼠脊髓损伤后室管膜中的细胞增殖及巢蛋白表达情况。
方法
采用动脉瘤夹压迫法建立大鼠脊髓损伤模型，通过病理及免疫组织化学染色显示不同时间室管膜细胞的巢蛋白表达及增殖情况。
结果
压迫后24小时，损伤部位附近的室管膜细胞巢蛋白表达显著增加。增殖细胞核抗原呈阳性，7天后观察到明显的增殖。随着时间推移，巢蛋白表达下调。
结论
正常情况下静止的成熟室管膜细胞在脊髓损伤后似乎会恢复到胚胎状态。
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