Agca Yuksel, Critser John K
Comparative Medicine Center, Research Animal Diagnostic Laboratory, University of Missouri-Columbia, Columbia, Missouri 65211, USA.
Semin Reprod Med. 2002 Feb;20(1):15-23. doi: 10.1055/s-2002-23516.
Spermatozoa were the first cells to be cryopreserved over 50 years ago, following the serendipitous discovery of the cryoprotective compound glycerol. This pioneering work was followed by the introduction of a series of other cryoprotectant chemicals referred to collectively as cryoprotective agents. Glycerol has been widely used in the cryopreservation of bull and human spermatozoa, although results are still highly variable across species as well as among individuals within a species. Recently, significant information has been gained with regard to the fundamental cryobiology of several mammalian species' spermatozoa that can be used to reduce this variability and develop improved methods for cryopreservation. In this concise review, we will discuss the fundamental cryobiology of cells in general and of mammalian spermatozoa in particular.
50多年前，在偶然发现冷冻保护化合物甘油之后，精子成为了首批被冷冻保存的细胞。在这项开创性工作之后，又引入了一系列其他冷冻保护化学物质，统称为冷冻保护剂。甘油已广泛用于牛和人类精子的冷冻保存，尽管不同物种以及同一物种内的个体之间的结果仍存在很大差异。最近，关于几种哺乳动物精子的基础低温生物学已经获得了重要信息，这些信息可用于减少这种变异性并开发改进的冷冻保存方法。在这篇简要综述中，我们将讨论一般细胞尤其是哺乳动物精子的基础低温生物学。
