Segawa K, Ikehara K, Okada Y
J Biochem. 1975 Jul;78(1):1-7.
A-Protein was isolated from a purified male-specific filamentous phage fd particle. A-Protein has a molecular weight of approximately 60,000 daltons, as estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The amino-terminal residue was glycine as determined by the dansylation technique. Amino acid analysis showed that histidine, arginine, and cysteine, which are not contained in B-protein, are present in A-protein.
A蛋白是从纯化的雄性特异性丝状噬菌体fd颗粒中分离出来的。通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳估计，A蛋白的分子量约为60,000道尔顿。通过丹磺酰化技术测定，氨基末端残基为甘氨酸。氨基酸分析表明，A蛋白中存在B蛋白中不含有的组氨酸、精氨酸和半胱氨酸。
