Rapaport Doron
Institut für Physiologische Chemie der Universität München Butenandtstr. 5, Haus B D-81377, München, Germany.
Trends Biochem Sci. 2002 Apr;27(4):191-7. doi: 10.1016/s0968-0004(02)02065-0.
The translocase at the outer membrane of mitochondria (TOM complex) mediates the initial steps of the import of preproteins into the organelle, which are essential for mitochondrial biogenesis and, therefore, for eukaryotic cell viability. The TOM complex is a multisubunit molecular machine with a dynamic structure. The biogenesis of TOM is of special interest because the complex is required for its own biogenesis. This article describes the mechanisms by which Tom components are targeted to the mitochondria and inserted into the outer membrane. The assembly of newly synthesized subunits into the functional TOM complex might occur via assembly intermediates that are in equilibrium with the mature complex.
线粒体外膜转位酶（TOM复合体）介导前体蛋白进入该细胞器的初始步骤，这对于线粒体生物合成乃至真核细胞的生存能力至关重要。TOM复合体是一种具有动态结构的多亚基分子机器。TOM的生物合成特别引人关注，因为该复合体自身的生物合成需要它。本文描述了Tom组分靶向线粒体并插入外膜的机制。新合成的亚基组装成功能性TOM复合体可能是通过与成熟复合体处于平衡状态的组装中间体来实现的。
