Teissié Justin
IPBS CNRS (UMR 5089) 205 route de Narbonne, 31077 Toulouse, France.
Cell Mol Biol Lett. 2002;7(1):96-100.
The cell membrane can be permeabilized when subjected to calibrated short electric pulses. This membrane alteration can be reversible, leaving cell viability unaffected. This set of events is called Electropermeabilization. This is now used in clinical applications to introduce hydrophilic drugs into the cytoplasm. While an empirical control of the electrical parameters is obtained, our knowledge of the molecular mechanisms supporting the membrane alteration is very limited. This paper gives a critical review of the limits of the models which are proposed by taking into account the different experimental approaches.
当受到校准的短电脉冲作用时，细胞膜会发生通透性改变。这种膜改变可以是可逆的，不会影响细胞活力。这一系列事件被称为电穿孔。目前它被用于临床应用中，将亲水性药物导入细胞质。虽然已经获得了对电参数的经验性控制，但我们对支持膜改变的分子机制的了解非常有限。本文通过考虑不同的实验方法，对所提出模型的局限性进行了批判性综述。
