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电穿孔所需的信号产生技术。电穿孔设备综述。

Techniques of signal generation required for electropermeabilization. Survey of electropermeabilization devices.

作者信息

Puc Marko, Corović Selma, Flisar Karel, Petkovsek Marko, Nastran Janez, Miklavcic Damijan

机构信息

University of Ljubljana, Faculty of Electrical Engineering, Trzaska 25, SI-1000, Slovenia.

出版信息

Bioelectrochemistry. 2004 Sep;64(2):113-24. doi: 10.1016/j.bioelechem.2004.04.001.

Abstract

Electropermeabilization is a phenomenon that transiently increases permeability of the cell plasma membrane. In the state of high permeability, the plasma membrane allows ions, small and large molecules to be introduced into the cytoplasm, although the cell plasma membrane represents a considerable barrier for them in its normal state. Besides introduction of various substances to cell cytoplasm, permeabilized cell membrane allows cell fusion or insertion of proteins to the cell membrane. Efficiency of all these applications strongly depends on parameters of electric pulses that are delivered to the treated object using specially developed electrodes and electronic devices--electroporators. In this paper we present and compare most commonly used techniques of signal generation required for electropermeabilization. In addition, we present an overview of commercially available electroporators and electroporation systems that were described in accessible literature.

摘要

电通透化是一种使细胞质膜通透性短暂增加的现象。在高通透性状态下,质膜允许离子、小分子和大分子进入细胞质,尽管细胞质膜在正常状态下对它们来说是一个相当大的屏障。除了将各种物质引入细胞质外,通透化的细胞膜还允许细胞融合或蛋白质插入细胞膜。所有这些应用的效率在很大程度上取决于使用专门开发的电极和电子设备——电穿孔仪传递到被处理对象的电脉冲参数。在本文中,我们介绍并比较了电通透化所需的最常用信号产生技术。此外,我们还概述了可获取文献中描述的市售电穿孔仪和电穿孔系统。

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