Kania B F, Matczuk J, Romanowicz K, Buéno L, Fioramonti J, Kania K
Department of Physiology, Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland.
Pol J Vet Sci. 2002;5(1):35-42.
The aim of this study was to determine the influence of mechanically induced duodenal distension (DD) and lorglumide (CCK1 receptor antagonist) premedication on electrical activity of various parts of gastrointestinal (GI) tract and the blood plasma cortisol level in sheep. The influence of lorglumide on the unfavourable effects of duodenal distension (performed with a balloon filled with water--40 and 80 ml; DD40 and DD80) was investigated in this study. These effects in sheep were as follows: the atony of forestomachs and abomasum and the transitory stimulation of myoelectrical activity of small intestine and distal parts of large intestine. The animals, under general anaesthesia, had electrodes inserted into the muscular layers of the organ, the duodenal fistula and (in another group of animals) also the ruminal fistula. Five minute duodenal distension (DD40 and DD80) caused an immediate and complete inhibition of the frequency of spike bursts as well as reticulo-ruminal and abomasal contractions, but also a transitory significant increase of spike bursts of the intestinal wall. The duodenal distension (DD40 and DD80) caused a significant increase of plasma cortisol concentration. Lorglumide did not significantly change the motility of gastrointestinal tract and cortisol concentration, but 10 min after the intracerebroventricular (i.c.v.) infusion in the doses of 1 and 2 mg in toto (i.e. 25 and 50 micrograms/kg B.W.) it decreased the cortisol concentration by 59.7%, as compared with the control values. Lorglumide administered in the above mentioned doses 10 min before the DD40 prevented all signs of intestinal stress and decreased the release of cortisol, but only for 10 min since the beginning of the duodenal distension. It is concluded, that lorglumide--an antagonist of the central CCK1 receptors can be an effective antistressoric agent in the stomach atony caused by the duodenal distension (mechanical-algetic-emotional stress) in sheep.
本研究的目的是确定机械性诱导的十二指肠扩张（DD）和洛谷胺（CCK1受体拮抗剂）预处理对绵羊胃肠道各部位电活动及血浆皮质醇水平的影响。本研究考察了洛谷胺对十二指肠扩张（用充满40和80毫升水的球囊进行——DD40和DD80）不利影响的作用。绵羊的这些影响如下：前胃和皱胃弛缓以及小肠和大肠远端部分肌电活动的短暂刺激。在全身麻醉下，将电极插入器官的肌层、十二指肠瘘管（在另一组动物中）以及瘤胃瘘管。五分钟的十二指肠扩张（DD40和DD80）导致棘波爆发频率以及网瘤胃和皱胃收缩立即完全抑制，但也导致肠壁棘波爆发短暂显著增加。十二指肠扩张（DD40和DD80）导致血浆皮质醇浓度显著升高。洛谷胺并未显著改变胃肠道的运动性和皮质醇浓度，但在以1和2毫克总量（即25和50微克/千克体重）进行脑室内（i.c.v.）输注10分钟后，与对照值相比，其使皮质醇浓度降低了59.7%。在DD40前10分钟给予上述剂量的洛谷胺可预防所有肠道应激迹象并降低皮质醇释放，但仅在十二指肠扩张开始后的10分钟内有效。得出的结论是，洛谷胺——一种中枢CCK1受体拮抗剂，在绵羊因十二指肠扩张（机械性-痛觉性-情绪性应激）引起的胃弛缓中可能是一种有效的抗应激剂。
