用于诊断登革热的各种商业检测方法的比较评估。

Comparative evaluation of various commercial assays for diagnosis of dengue fever.

作者信息

Vajpayee M, Singh U B, Seth P, Broor S

机构信息

Department of Microbiology, All India Institute of Medical Sciences, New Delhi.

出版信息

Southeast Asian J Trop Med Public Health. 2001 Sep;32(3):472-5.

PMID:11944701

Abstract

Dengue fever (DF) is endemic in India and dengue hemorrhagic fever (DHF) has been reported with increasing frequency in the last decade. We evaluated three commercial assays for detection of antibodies to dengue virus, to assess their performance in a diagnostic laboratory. Sera from 58 patients collected during a febrile outbreak in New Delhi in 1997 were studied. The methods evaluated were MRL Diagnostic Dengue Fever Virus IgM Capture ELISA, Pan Bio Dengue Duo IgM and IgG Capture ELISA and Pan Bio Rapid Immunochromatographic test. The MRL ELISA correctly identified 97.8% (43 of 44) of samples as dengue positive while the Pan Bio Duo ELISA and Pan Bio RIT identified 95.45% (42 of 44). The sensitivities of both Pan Bio Duo ELISA and Pan Bio RIT for primary dengue and secondary dengue were 100% and 93.54% respectively. The specificity of three assays were MRL IgM ELISA 100%, Pan Bio Duo ELISA 92.8% and Pan Bio RIT 85.7%.

摘要

登革热（DF）在印度呈地方性流行，并且在过去十年中，登革出血热（DHF）的报告频率不断增加。我们评估了三种用于检测登革热病毒抗体的商业检测方法，以评估它们在诊断实验室中的性能。对1997年新德里一次发热疫情期间收集的58例患者的血清进行了研究。评估的方法有MRL诊断登革热病毒IgM捕获ELISA、泛生物登革热双抗原IgM和IgG捕获ELISA以及泛生物快速免疫色谱检测。MRL ELISA正确地将97.8%（44例中的43例）的样本鉴定为登革热阳性，而泛生物双抗原ELISA和泛生物快速免疫色谱检测分别鉴定出95.45%（44例中的42例）。泛生物双抗原ELISA和泛生物快速免疫色谱检测对原发性登革热和继发性登革热的敏感性分别为100%和93.54%。三种检测方法的特异性分别为：MRL IgM ELISA为100%，泛生物双抗原ELISA为92.8%，泛生物快速免疫色谱检测为85.7%。