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采用膜片钳技术研究磁场暴露对钙通道电流的影响。

Effect of magnetic field exposure on calcium channel currents using patch clamp technique.

作者信息

Obo Mayumi, Konishi Shiro, Otaka Yoshihisa, Kitamura Satoshi

机构信息

Mitsubishi Chemical Safety Institute, Ibaraki, Japan.

出版信息

Bioelectromagnetics. 2002 May;23(4):306-14. doi: 10.1002/bem.10022.

DOI:10.1002/bem.10022
PMID:11948611
Abstract

Calcium influxes through the membrane of PC-12D cells were measured under exposure to DC biased AC magnetic fields in resonant conditions of the ion cyclotron and the ion parametric resonance hypotheses and compared with influxes in cells without exposure to the magnetic field. After cancellation of the geomagnetic field, the cells were exposed to the horizontal fields generated by a current sheet, a planar sheet of conductor which generated a satisfactorily homogeneous horizontal magnetic field on the stage of a microscope without hindering treatment of a cell under observation. At or near any resonant conditions, no change in calcium influx could be detected under standard patch clamp conditions.

摘要

在离子回旋共振和离子参量共振假说的共振条件下,测量暴露于直流偏置交流磁场中的PC - 12D细胞膜上的钙内流,并与未暴露于磁场的细胞中的钙内流进行比较。在地磁场消除后,将细胞暴露于由电流片产生的水平磁场中,电流片是一种平面导体片,在显微镜载物台上产生了令人满意的均匀水平磁场,且不妨碍对观察中的细胞进行处理。在任何共振条件或接近共振条件下,在标准膜片钳条件下未检测到钙内流的变化。

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