Yang Byung Yun, Ding Qiong, Montgomery Rex
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City, IA 52242, USA.
Carbohydr Res. 2002 Apr 17;337(8):731-42. doi: 10.1016/s0008-6215(02)00042-3.
Extracellular polysaccharides (EPSs) produced by an Erwinia sp associated with a fungal canker disease of Eucalyptus were fractionated into one polysaccharide that was identified with that produced by Erwinia chrysanthemi strains SR260, Ech1, and Ech9, and the other distinctively different from any other EPS produced by E. chrysanthemi strains so far studied. Their structures were determined using a combination of chemical and physical techniques including methylation analysis, low pressure gel-filtration, and anion-exchange chromatographies, high-pH anion-exchange chromatography, mass spectrometry and 1D and 2D 1H NMR spectroscopy. The new polysaccharide, identified as EPS Teranera, has the following structure: [structure: see text] The molecular weights of the polysaccharides range from 3.2-6.2 x 10(5) and their hydrodynamic properties are those of polydisperse, polyanionic biopolymers with pseudoplastic, non-thixotropic flow characteristics in aqueous solutions.
与桉树真菌性溃疡病相关的一种欧文氏菌产生的胞外多糖（EPSs）被分离成一种多糖，该多糖与菊欧文氏菌菌株SR260、Ech1和Ech9产生的多糖相同，另一种则与迄今为止研究的菊欧文氏菌菌株产生的任何其他EPS明显不同。使用化学和物理技术相结合的方法确定了它们的结构，这些技术包括甲基化分析、低压凝胶过滤、阴离子交换色谱、高pH值阴离子交换色谱、质谱以及一维和二维氢核磁共振光谱。新的多糖被鉴定为EPS Teranera，其结构如下：[结构：见原文] 这些多糖的分子量范围为3.2 - 6.2×10⁵，其流体动力学性质为多分散、聚阴离子生物聚合物，在水溶液中具有假塑性、非触变性流动特性。
