Chen Z Y, Cao L, Lu C L, He C, Bao X
Department of Neurobiology, Second Military Medical University, Shanghai 200433, China.
Sheng Li Xue Bao. 2000 Aug;52(4):295-300.
The aim of the study was to investigate the effect of exogenous glial cell line derived neurotrophic factor (GDNF) on spinal cord neurons after sciatic nerve axotomy. Upon silicone tubulization of transected sciatic nerve in the adult rat, either 0.9% saline or GDNF solution was injected into the silicone chamber. It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions. These results suggest that exogenous GDNF is capable of protecting motor neurons from death induced by peripheral nerve injury.
本研究的目的是探讨外源性胶质细胞源性神经营养因子（GDNF）对坐骨神经切断后脊髓神经元的影响。在成年大鼠横断坐骨神经的硅胶管植入术后，将0.9%生理盐水或GDNF溶液注入硅胶管腔。通过尼氏染色和酶组织化学染色观察到，外源性GDNF可减少脊髓前角外侧核损伤诱导的运动神经元死亡，以及脊髓和感觉神经节中胆碱酯酶和酸性磷酸酶活性的变化。这些结果表明，外源性GDNF能够保护运动神经元免受周围神经损伤诱导的死亡。
