This study investigates the effect of acute in vivo and in vitro ethanol administration on the contractions evoked electrically and by exogenous noradrenaline and alpha,beta-methylene-ATP in the rat bisected vas deferens. 2. In vivo ethanol treatment (3 g kg(-1), i.p.) significantly potentiated the early purinergic (phase I) and the delayed adrenergic (phase II) phases evoked by single-pulse stimulation of the epididymal portion of the rat vas deferens, leaving unaffected both phases in the prostatic portion. In vitro 50 mM ethanol significantly depressed phase I leaving unaffected phase II in both portions from untreated rats. In vitro ethanol significantly depressed phase I in the epididymal portion from in vivo ethanol treated animals and potentiated phase II in both portions. 3. In vivo ethanol treatment (3.0 g kg(-1), i.p.) selectively impaired the response to noradrenaline only in the prostatic portion of rat vas deferens while it was devoid of any action on alpha,beta-methylene-ATP contractions. Ethanol 50 mM in vitro was devoid of any action on the response to exogenous noradrenaline and alpha,beta-methylene-ATP in both tissues. 4. In vivo ethanol treatment slightly but significantly increased the phasic response in the epididymal portion to trains of stimuli (2-30 Hz). In vitro 50 mM ethanol was ineffective against the phasic and tonic contractions elicited by the tetanus in both portions. 5. It is concluded that ethanol treatment affects purinergic and adrenergic pathways of transmission possibly leading to a disruption of physiological contractions necessary to seminal emission.
摘要
本研究调查了急性体内和体外给予乙醇对大鼠离体输精管中电刺激、外源性去甲肾上腺素和α,β-亚甲基三磷酸腺苷（α,β-methylene-ATP）诱发的收缩的影响。2. 体内给予乙醇（3 g kg⁻¹，腹腔注射）显著增强了大鼠输精管附睾段单脉冲刺激诱发的早期嘌呤能（I期）和延迟肾上腺素能（II期）阶段，而前列腺段的这两个阶段不受影响。体外50 mM乙醇显著抑制了未处理大鼠两个部位的I期，而II期不受影响。体外乙醇显著抑制了体内乙醇处理动物附睾段的I期，并增强了两个部位的II期。3. 体内给予乙醇（3.0 g kg⁻¹，腹腔注射）仅选择性损害了大鼠输精管前列腺段对去甲肾上腺素的反应，而对α,β-亚甲基三磷酸腺苷诱发的收缩无任何作用。体外50 mM乙醇对两个组织对外源性去甲肾上腺素和α,β-亚甲基三磷酸腺苷的反应均无任何作用。4. 体内给予乙醇轻微但显著增加了附睾段对串刺激（2 - 30 Hz）的相位反应。体外50 mM乙醇对两个部位由强直刺激诱发的相位和强直收缩均无效。5. 结论是，乙醇处理影响嘌呤能和肾上腺素能传递途径，可能导致射精所需的生理收缩受到破坏。
