[卡介苗对大鼠哮喘模型制作的影响及其与γδT细胞关系的实验研究]

[An experimental study of the effect of bacille calmette-guerin vaccine on the production of a rat asthmatic model and its relation with gamma delta T cells].

作者信息

Li Chaoqian, Xu Yongjian, Zhang Zhenxiang, Yang Danlei, Liu Xiansheng, Xiong Weining

机构信息

Department of Respiratory Disease, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology, Wuhan 430030, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2002 Mar;25(3):162-5.

PMID:11953115

Abstract

OBJECTIVE

To explore the preventive effect of BCG vaccine on asthma attack in rats and its relation with gamma delta T cells.

METHODS

Wistar rats (n = 10, for each group) were immunized with BCG vaccine. After 8 weeks, the rats were sensitized and challenged with ovalbumin to establish an asthmatic model. After 3 weeks, the airway responsiveness (PC(50)) was determined. The percentage of gamma delta T cells or alpha beta T cells among total T cells or total lymphocytes and the ratio of the mean fluorescence density of CD(28)/gamma delta TCR of gamma delta T cell subset in peripheral blood and BALF were detected by flow cytometry, immunofluorescence and immunohistochemistry.

RESULTS

In the rat asthmatic model, the number of BALF eosinophils was increased [(2.5 +/- 1.1) x 10(8)/L vs (0.0) x 10(8)/L], (P < 0.01). while the PC(50) was decreased [(0.28 +/- 0.10) g/L vs (1.36 +/- 0.76) g/L], (P < 0.01). However, the results of the group immunized with BCG vaccine were same as the controls [EOS (0.0) x 10(8)/L vs (0.0) x 10(8)/L], (P > 0.05). PC(50) [(1.28 +/- 0.77) g/L vs (1.36 +/- 0.76) g/L, (P > 0.05)]. In the asthmatic group, gamma delta T cell/T cell was (32.4 +/- 2.6)%, and gamma delta T cell/lymphocytes (28.6 +/- 2.4)%. In the group immunized with BCG vaccine, gamma delta T cell/T cell was (41.3 +/- 6.0)% and gamma delta T cell/lymphocytes (35.2 +/- 3.3)%. In the asthmatic group immunized with BCG vaccine, gamma delta T cell/T cell was (47.3 +/- 8.5)% and gamma delta T cell/lymphocytes (39.0 +/- 6.8)%. In the control group, gamma delta T cell/T cell was (27.3 +/- 0.8)% and gamma delta T cell/lymphocytes (21.8 +/- 1.9)%, (all P < 0.01). The ratio of the mean fluorescence density of CD(28)/gamma delta TCR of gamma delta T cell subset (0.66 +/- 0.08, 0.88 +/- 0.26, 0.71 +/- 0.15 vs 0.53 +/- 0.06, (all P < 0.01) were increased.

CONCLUSIONS

BCG vaccine was shown to be able to prevent the production of a rat asthmatic model. gamma delta T cells may also have a Th1/Th2 profile, and these cells may be important in the immunoregulation of BCG vaccine and the pathogenesis of asthma.

摘要

目的

探讨卡介苗对大鼠哮喘发作的预防作用及其与γδT细胞的关系。

方法

将Wistar大鼠（每组10只）用卡介苗免疫。8周后，用卵清蛋白对大鼠进行致敏和激发以建立哮喘模型。3周后，测定气道反应性（PC50）。采用流式细胞术、免疫荧光和免疫组织化学检测外周血和支气管肺泡灌洗液中总T细胞或总淋巴细胞中γδT细胞或αβT细胞的百分比以及γδT细胞亚群CD28/γδTCR的平均荧光密度比值。

结果

在大鼠哮喘模型中，支气管肺泡灌洗嗜酸粒细胞数量增加[（2.5±1.1）×10⁸/L对（0.0）×10⁸/L]，（P<0.01），而PC50降低[（0.28±0.10）g/L对（1.36±0.76）g/L]，（P<0.01）。然而，卡介苗免疫组的结果与对照组相同[嗜酸粒细胞（0.0）×10⁸/L对（0.0）×10⁸/L]，（P>0.05），PC50[（1.28±0.77）g/L对（1.36±0.76）g/L，（P>0.05）]。在哮喘组中，γδT细胞/T细胞为（32.4±2.6）%，γδT细胞/淋巴细胞为（28.6±2.4）%。在卡介苗免疫组中，γδT细胞/T细胞为（41.3±6.0）%，γδT细胞/淋巴细胞为（35.2±3.3）%。在卡介苗免疫的哮喘组中，γδT细胞/T细胞为（47.3±8.5）%，γδT细胞/淋巴细胞为（39.0±6.8）%。在对照组中，γδT细胞/T细胞为（27.3±0.8）%，γδT细胞/淋巴细胞为（21.8±1.9）%，（均P<0.01）。γδT细胞亚群CD28/γδTCR的平均荧光密度比值（0.66±0.08、0.88±0.26、0.71±0.15对0.53±0.06，均P<0.01）升高。