外源性葡萄糖对曼氏血吸虫尾蚴存活及感染力的影响。
Effects of exogenous glucose on survival and infectivity of Schistosoma mansoni cercariae.
作者信息
Fried Bernard, Laterra Robert, Kim Yonghyun
机构信息
Department of Biology, Lafayette College, Easton, PA 18042, USA.
出版信息
Korean J Parasitol. 2002 Mar;40(1):55-8. doi: 10.3347/kjp.2002.40.1.55.
The effects of exogenous glucose in artificial spring water (ASW) were studied on the survival and infectivity of Schistosoma mansoni cercariae. The mean percent survival of cercariae maintained in 1% glucose in ASW for 36 and 48 hr was significantly greater than that of cercariae maintained identically in ASW. Cercariae maintained in ASW with or without glucose for 24 hr, fixed in neutral buffered formalin, and stained in Oil Red O, showed an accumulation of neutral lipid in the tail. Cercariae maintained as described above and stained in periodic acid-Schiff exhibited depleted glycogen, mainly from the tail. Cercariae maintained in ASW with glucose for 24 hr did not resynthesize glycogen. Cercariae maintained in ASW with glucose for 24 hr were as capable of infecting male FVBN202 mice as were freshly emerged cercariae, and increased the percent of worm recovery. Exogeneous glucose added to ASW prolonged the survival of S. mansoni cercariae and increased infectivity in terms of worm recovery.
研究了人工泉水中外源性葡萄糖对曼氏血吸虫尾蚴存活和感染力的影响。在人工泉水中1%葡萄糖中维持36和48小时的尾蚴平均存活百分比显著高于在人工泉水中相同条件下维持的尾蚴。在含或不含葡萄糖的人工泉水中维持24小时、用中性缓冲福尔马林固定并用油红O染色的尾蚴，尾部出现中性脂质积累。按上述方法维持并用过碘酸希夫染色的尾蚴糖原减少，主要来自尾部。在含葡萄糖的人工泉水中维持24小时的尾蚴不能重新合成糖原。在含葡萄糖的人工泉水中维持24小时的尾蚴与刚逸出的尾蚴一样能够感染雄性FVBN202小鼠，并提高了虫体回收率。添加到人工泉水中的外源性葡萄糖延长了曼氏血吸虫尾蚴的存活时间，并在虫体回收率方面提高了感染力。
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