Yue Lin, Qiu Lihui, Gao Xuejun
Department of Operative Dentistry, Cariology and Endodontology, Peking University School of Stomatology, Beijing 100081, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2002 Jan;37(1):39-42.
To investigate the relationship of the levels of proteins in parotid saliva, whole saliva and dental plaque fluid with caries susceptibility.
Sixty-six of university students were selected as subjects, 39 in caries-free group (CF, DMFS = 0) and 27 in caries-susceptible group (CS, DMFS >/= 8 and DT >/= 3). Total protein concentration was detected with Lowry method. Protein compositions were separated with SDS-PAGE and alkaline electrophoresis. The gels were analyzed using an image evaluation system.
Total protein level in dental plaque fluid was about 10-fold higher than that in saliva. Whole saliva was closely related to dental plaque fluid in terms of proteins only in CF group (r = 0.804), but there was little relation in CS group. The proteins that occurred in all three fluids were 14 000, 66 000 and 76 000 proteins. The 14 000, 15 000 and 38 000 proteins level in dental plaque fluid and 14 000 protein level in whole saliva were significantly lower in CS group than in CF group.
The proteins of dental plaque fluid are influenced significantly by whole saliva in CF group. The results suggest some kinds of proteins in dental plaque fluid and in whole saliva might play important roles against caries.
探讨腮腺唾液、全唾液及牙菌斑液中蛋白质水平与龋齿易感性的关系。
选取66名大学生作为研究对象，其中无龋组39人（CF，DMFS = 0），龋易感组27人（CS，DMFS≥8且DT≥3）。采用Lowry法检测总蛋白浓度。用SDS-PAGE和碱性电泳分离蛋白质成分。使用图像评估系统对凝胶进行分析。
牙菌斑液中的总蛋白水平约为唾液中的10倍。仅在无龋组中，全唾液与牙菌斑液在蛋白质方面密切相关（r = 0.804），而在龋易感组中关系不大。在所有三种液体中都存在的蛋白质是14000、66000和76000的蛋白质。龋易感组牙菌斑液中14000、15000和38000的蛋白质水平以及全唾液中14000的蛋白质水平均显著低于无龋组。
在无龋组中，全唾液对牙菌斑液中的蛋白质有显著影响。结果表明，牙菌斑液和全唾液中的某些蛋白质可能在预防龋齿方面发挥重要作用。
