Magiorkinis E, Paraskevis D, Magiorkinis G, Chryssou S, Chini M, Lazanas M, Paparizos V, Saroglou G, Antoniadou A, Giamarellou E, Karafoulidou A, Hatzakis A
Department of Hygiene and Epidemiology, National Retrovirus Reference Center, Athens School of Medicine, University of Athens, Alexanfroupoles 25, GR-115 27, Greece.
Virus Res. 2002 Apr 23;85(1):109-15. doi: 10.1016/s0168-1702(02)00022-9.
The widespread use of antiviral drugs against HIV has increased the prevalence of HIV-1 resistant strains among naïve individuals due to transmission of resistant strains. The purpose of this study was to investigate the presence of HIV-1 strains harboring resistance mutations in naïve patients in Greece. Blood samples were collected from 25 individuals. The DNA sequence of protease and partial reverse transcriptase regions (codons 41-223) were obtained by direct sequencing. Our results showed the absence of any primary resistance mutations in the study population. However, we were able to identify high prevalence of sequence polymorphisms at positions in reverse transcriptase region associated mainly with resistance to NNRTIs. Moreover, in protease region several secondary mutations were detected, suggesting the higher genetic variability of this region. The clinical significance of the polymorphisms associated with reduced susceptibility to NNRTIs remains to be clarified.
由于耐药毒株的传播，抗HIV抗病毒药物的广泛使用增加了初治个体中HIV-1耐药毒株的流行率。本研究的目的是调查希腊初治患者中携带耐药突变的HIV-1毒株的存在情况。采集了25名个体的血样。通过直接测序获得蛋白酶和部分逆转录酶区域（密码子41-223）的DNA序列。我们的结果显示研究人群中不存在任何原发性耐药突变。然而，我们能够识别出逆转录酶区域中主要与对非核苷类逆转录酶抑制剂（NNRTIs）耐药相关位置的序列多态性高流行率。此外，在蛋白酶区域检测到了几个继发性突变，表明该区域具有更高的遗传变异性。与对NNRTIs敏感性降低相关的多态性的临床意义仍有待阐明。
