Olsson Pär, Collins V Peter, Liu Lu, Gedda Lars, Liljegren Asa, Carlsson Jörgen
Division of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden.
Int J Oncol. 2002 May;20(5):1057-63.
Chinese hamster ovary (CHO) cells transfected with the wild-type gene for the human epidermal growth factor-receptor (EGFR) and expressing the receptor in their cell membrane are, together with the receptor negative parent CHO cells, an interesting model system for experimental EGFR-targeting tumour therapy. Comparisons of effects on nearly identical cells with and without receptors can be made. The main purpose of this work was to compare the internalisation and retention of the radioactivity delivered as 125I-EGF or 125I-EGF-dextran in transfected cells (called CHO-EGFR), and human glioma cells U-343 which naturally express wild-type EGFR. We found that radioactivity delivered as 125I-EGF-dextran was retained intracellularly by both cell types to a higher degree than radioactivity delivered as 125I-EGF. Prolonging the cellular exposure time for 125I-EGF-dextran considerably increased postincubation intracellular retention in both cell types. No major differences between the two EGFR expressing cell lines were found and, based on the results in this work, CHO-EGFR cells seem an adequate model for experiments with agents targeting the EGF-receptor.
用人类表皮生长因子受体（EGFR）的野生型基因转染并在细胞膜上表达该受体的中国仓鼠卵巢（CHO）细胞，与受体阴性的亲本CHO细胞一起，是用于实验性EGFR靶向肿瘤治疗的一个有趣的模型系统。可以对有受体和无受体的几乎相同的细胞的效应进行比较。这项工作的主要目的是比较以¹²⁵I-表皮生长因子（¹²⁵I-EGF）或¹²⁵I-EGF-葡聚糖形式递送的放射性在转染细胞（称为CHO-EGFR）和天然表达野生型EGFR的人类胶质瘤细胞U-343中的内化和保留情况。我们发现，以¹²⁵I-EGF-葡聚糖形式递送的放射性在两种细胞类型中的细胞内保留程度均高于以¹²⁵I-EGF形式递送的放射性。延长¹²⁵I-EGF-葡聚糖的细胞暴露时间会显著增加两种细胞类型在孵育后细胞内的保留量。在两种表达EGFR的细胞系之间未发现重大差异，基于这项工作的结果，CHO-EGFR细胞似乎是用于EGF受体靶向药物实验的合适模型。
