拓扑异构酶IIα作为脑膜瘤中一种可靠的增殖标志物。

Topoisomerase II alpha as a reliable proliferation marker in meningiomas.

作者信息

Roessler Karl, Gatterbauer Brigitte, Kitz Klaus

机构信息

Department of Neurosurgery, University of Vienna Medical School, Austria.

出版信息

Neurol Res. 2002 Apr;24(3):241-3. doi: 10.1179/016164102101199855.

DOI:10.1179/016164102101199855

PMID:11958416

Abstract

The value of DNA Topoisomerase IIalpha expression as a proliferation marker in meningiomas was investigated. The correlation to MIB-1 expression and tumor grade in 85 meningiomas (60 classical, 19 atypical and 6 anaplastic) was analysed by immunohistochemistry. MIB-1 labeling indices (LI) were 1.1% (+/- 0.85) for classical meningiomas, 1.9% (+/- 1.5) for atypical meningiomas and 5.6% (+/- 4.1) for anaplastic meningiomas, differences statistically significant (p = 0.03, < 0.0001, < 0.0001). Topoisomerase IIalpha LIs were 1.4% (+/- 1.2) for classical, 3.2% (+/- 2.4) for atypical and 9.7% (+/- 6.6) for anaplastic meningiomas, differences statistically significant (p = 0.003, < 0.0001, < 0.0001). Proliferation indices based on cells staining for MIB-1 and Topoisomerase IIalpha correlated highly with one another (r= 0.906, p <0.0001). Topoisomerase IIalpha exhibited a more distinct, less variable nuclear staining pattern compared to MIB-1 expression. DNA Topoisomerase IIalpha LI represents a reliable alternative to MIB-1 as a proliferation marker in human meningiomas, especially for computer assisted assessment, where a distinct uniform staining pattern is required.

摘要

研究了DNA拓扑异构酶IIα表达作为脑膜瘤增殖标志物的价值。通过免疫组织化学分析了85例脑膜瘤（60例经典型、19例非典型和6例间变型）中其与MIB-1表达及肿瘤分级的相关性。经典型脑膜瘤的MIB-1标记指数（LI）为1.1%（±0.85），非典型脑膜瘤为1.9%（±1.5），间变型脑膜瘤为5.6%（±4.1），差异具有统计学意义（p = 0.03，<0.0001，<0.0001）。拓扑异构酶IIα的LI在经典型脑膜瘤中为1.4%（±1.2），非典型脑膜瘤中为3.2%（±2.4），间变型脑膜瘤中为9.7%（±6.6），差异具有统计学意义（p = 0.003，<0.0001，<0.0001）。基于MIB-1和拓扑异构酶IIα染色细胞的增殖指数彼此高度相关（r = 0.906，p <0.0001）。与MIB-1表达相比，拓扑异构酶IIα表现出更明显、变异性更小的核染色模式。DNA拓扑异构酶IIα的LI作为人脑膜瘤增殖标志物是MIB-1的可靠替代指标，尤其适用于需要明显均匀染色模式的计算机辅助评估。