Taniguchi K, Wakabayashi T, Yoshida T, Mizuno M, Yoshikawa K, Kikuchi A, Nakashima N, Yoshida J
Department of Neurosurgery and Laboratory of Medical Mycology, Research Institute of Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
J Neurosurg. 1999 Sep;91(3):477-82. doi: 10.3171/jns.1999.91.3.0477.
The enzyme DNA topoisomerase IIalpha (Topo IIalpha) was tested as a measure of cell proliferation in gliomas.
Immunostaining for the Topo IIalpha and for the Ki-67 antigen (MIB-1 antibody) was performed in paraffin-embedded tissue sections obtained from 25 resected human gliomas. Additionally, cultured human glioma cells were subjected to simultaneous flow cytometry to determine Topo IIalpha and DNA content. Using flow cytometric analysis, the authors found that the Topo IIalpha antibody labeled cells in the S, G2, and M phases of the cell cycle and also those in some parts of the G0 and G1 phases. In histological sections, Topo IIalpha showed more distinct staining than MIB-1, particularly in older archival cases. The proliferative indices (PIs) based on cells staining for MIB-1 and Topo IIalpha correlated highly with one another (r = 0.96). The Topo IIalpha PI immunopositivity was seen in 4.07% of cells in the low-grade astrocytoma group, 11.97% in the anaplastic astrocytoma group, and 13.84% in the glioblastoma multiforme group, representing significant differences between low-grade astrocytoma and both anaplastic astrocytoma and glioblastoma. A Topo IIalpha PI less than 5% predicted longer patient survival (p = 0.003).
Immunostaining for Topo IIalpha represents a useful alternative to MIB-1 as a proliferative index in human gliomas.
检测DNA拓扑异构酶IIα（Topo IIα）作为胶质瘤细胞增殖的一项指标。
对取自25例手术切除的人脑胶质瘤石蜡包埋组织切片进行Topo IIα和Ki-67抗原（MIB-1抗体）免疫染色。此外，对培养的人脑胶质瘤细胞进行同步流式细胞术检测，以确定Topo IIα和DNA含量。通过流式细胞术分析，作者发现Topo IIα抗体标记细胞周期S期、G2期和M期的细胞，以及G0期和G1期部分细胞。在组织学切片中，Topo IIα染色比MIB-1更明显，尤其是在存档时间较长的病例中。基于MIB-1和Topo IIα染色细胞的增殖指数（PI）彼此高度相关（r = 0.96）。低级别星形细胞瘤组4.07%的细胞出现Topo IIα PI免疫阳性，间变性星形细胞瘤组为11.97%，多形性胶质母细胞瘤组为13.84%，这表明低级别星形细胞瘤与间变性星形细胞瘤和多形性胶质母细胞瘤之间存在显著差异。Topo IIα PI小于5%预示患者生存期更长（p = 0.003）。
Topo IIα免疫染色可作为MIB-1的一种有用替代方法，用于评估人脑胶质瘤的增殖指数。
