DeGiorgio Lorraine A, Manuelidis Laura, Bernstein Jerald J
Department of Neurology and Neuroscience, Weill College of Medicine of Cornell University at the Burke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605, USA.
Neurosci Lett. 2002 Mar 29;322(1):62-6. doi: 10.1016/s0304-3940(02)00065-4.
Cells cultured from Alzheimer disease leptomeninges or skin were grafted into the cortex of adult thymectomized rats. At 3 days post-implant, plaque-like aggregates were found in the cortex, corpus callosum, septum and caudate nucleus. These structures were immunopositive for human amyloid precursor protein (APP), human amyloid beta peptide (Abeta), cathepsin D, apolipoprotein E and ubiquitin. Aberrant tau+ neurites, reactive astrocytes and microglia were associated with many aggregates. Although birefringent amyloid occupied the central area of most aggregates, these structures had disappeared by l month post-implant. Abeta and APP produced by grafted non-neural human cells can penetrate rat brain and form plaque-like structures, which can be effectively cleared by the rat.
从阿尔茨海默病软脑膜或皮肤培养的细胞被移植到成年去胸腺大鼠的皮质中。植入后3天，在皮质、胼胝体、隔区和尾状核中发现了斑块样聚集物。这些结构对人淀粉样前体蛋白（APP）、人淀粉样β肽（Abeta）、组织蛋白酶D、载脂蛋白E和泛素呈免疫阳性。异常的tau+神经突、反应性星形胶质细胞和小胶质细胞与许多聚集物相关。尽管双折射淀粉样蛋白占据了大多数聚集物的中心区域，但这些结构在植入后1个月时已消失。移植的非神经人类细胞产生的Abeta和APP可以穿透大鼠大脑并形成斑块样结构，而大鼠可以有效地清除这些结构。
