牛晶状体醛糖还原酶在特定测定条件下的动力学行为。

Kinetic behaviour under defined assay conditions for bovine lens aldose reductase.

作者信息

Crabbe M J, Halder A B

出版信息

Clin Biochem. 1979 Dec;12(6):281-3. doi: 10.1016/s0009-9120(79)80129-0.

PMID:119591

Abstract

Bovine lens aldose reductase, an enzyme important in sugar cataract formation, has been purified using a new procedure, and its optimum conditions of assay defined in both lens homogenates and purified preparations. Preliminary kinetic analysis shows that the degree of the rate equation is of a low order, and may be Michaelian under the standard conditions used.

摘要

牛晶状体醛糖还原酶是一种在糖性白内障形成中起重要作用的酶，已采用一种新方法进行了纯化，并在晶状体匀浆和纯化制剂中确定了其最佳测定条件。初步动力学分析表明，速率方程的阶数较低，在所使用的标准条件下可能符合米氏方程。