Slavík R, Homola J, Brynda E
Institute of Radio Engineering and Electronics, AS CR, Chaberská 57, 182 51, Prague, Czech Republic.
Biosens Bioelectron. 2002 Jun;17(6-7):591-5. doi: 10.1016/s0956-5663(02)00013-1.
A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B (SEB) is reported. The sensor is based on spectral interrogation of surface plasmons in a miniature sensing element based on a side-polished single-mode optical fiber with a thin metal overlayer. For specific detection of SEB, the SPR sensor is functionalized with a covalently crosslinked double-layer of antibodies against SEB. The SPR biosensor is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min.
报道了一种用于检测葡萄球菌肠毒素B（SEB）的光纤表面等离子体共振（SPR）生物传感器。该传感器基于对基于侧面抛光单模光纤并带有薄金属覆盖层的微型传感元件中的表面等离子体进行光谱询问。为了特异性检测SEB，SPR传感器用针对SEB的共价交联双层抗体进行功能化。已证明该SPR生物传感器能够在不到10分钟的时间内检测出纳克/毫升浓度的SEB。
