Gower W R, Sanberg P R, Brown P G, Salyani S, Pasqual C J, McGrogan M, Good R A, Engelman R W
Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, James A. Haley Veterans Hospital, Tampa, FL 33612, USA.
J Neuroimmunol. 2002 Apr;125(1-2):103-13. doi: 10.1016/s0165-5728(02)00041-3.
Ntera2/D1 cells had an A1 B8 Bw6 Cw7 DR3 DR52 major histocompatibility complex (MHC) genotype. Its neuronal derivative, hNT neurons, expressed A1 B8 Bw6 MHC class I molecules, but did not activate, and its hNT supernatant suppressed allogeneic mixed lymphocyte cultures (MLC) >98% (p<0.01), phytohemagglutinin (PHA)-activated T-cell proliferation >87% (p<0.01), even 48 h after stimulation, suppressed phorbol 12-myristate 13-acetate (PMA)/ionomycin-induced T-cell proliferation >99% (p<0.001), and reduced interleukin-2 (IL-2) production (p<0.01), while maintaining T cells in a quiescent G(0)/G(1) state without lowering their viability. This immunosuppressive activity was attributed to a 40-100-kDa anionic hNT protein with an isoelectric point of 4.8.
Ntera2/D1细胞具有A1 B8 Bw6 Cw7 DR3 DR52主要组织相容性复合体（MHC）基因型。其神经衍生物hNT神经元表达A1 B8 Bw6 MHC I类分子，但不激活，且其hNT上清液抑制同种异体混合淋巴细胞培养（MLC）>98%（p<0.01），抑制植物血凝素（PHA）激活的T细胞增殖>87%（p<0.01），即使在刺激后48小时，抑制佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）/离子霉素诱导的T细胞增殖>99%（p<0.001），并减少白细胞介素-2（IL-2）的产生（p<0.01），同时使T细胞维持在静止的G(0)/G(1)状态而不降低其活力。这种免疫抑制活性归因于一种40-100 kDa的阴离子hNT蛋白，其等电点为4.8。
