Tan D Y, Caramelo C
Department of Pathophysiology, Jinan University Medical College, Guangzhou 510632, China.
Sheng Li Xue Bao. 2000 Apr;52(2):103-8.
In the present work, we have examined the effects of selective inhibition of the inducible isoform of nitric oxide synthase (iNOS) in rat medulla by aminoguanidine (AG) on the arterial pressure of Dahl salt sensitive (DS), Dahl salt-resistant (DR) and Sprague Dawley (SD) rats by chronic in vivo hemodynamic experiment, the effect of sodium chloride (NaCl) or NaCl plus AG infusion on urinary nitrate/nitrite (urinary NO3/NO2 UNO(x)), the end product of nitric oxide (NO), excretion, glomerular filtration rate (GFR), effective renal plasma flow (ERPF) and plasma renin activity (PRA). Furthermore, an iNOS activity assay was also made. The results showed that AG infusion significantly augmented the pressor response of DS and SD rats to high NaCl (8%) intake, and decreased GFR, ERPF and PRA of DS rats. In addition, in DS rats, renal medullary interstitial administration of high NaCl significantly elevated the iNOS activity of renal tissue, especially inner medulla and outer medulla, and greatly increased UNO(x) excretion. Therefore, it is concluded that inducible NOS is an important modulator of blood pressure in case of NaCl-induced hypertension.
在本研究中，我们通过慢性体内血流动力学实验，研究了用氨基胍（AG）选择性抑制大鼠延髓中诱导型一氧化氮合酶（iNOS）对 Dahl 盐敏感（DS）、Dahl 盐抵抗（DR）和 Sprague Dawley（SD）大鼠动脉血压的影响，以及输注氯化钠（NaCl）或 NaCl 加 AG 对尿硝酸盐/亚硝酸盐（尿 NO3/NO2，即 UNO(x)）排泄、肾小球滤过率（GFR）、有效肾血浆流量（ERPF）和血浆肾素活性（PRA）的影响。此外，还进行了 iNOS 活性测定。结果表明，输注 AG 显著增强了 DS 和 SD 大鼠对高 NaCl（8%）摄入的升压反应，并降低了 DS 大鼠的 GFR、ERPF 和 PRA。此外，在 DS 大鼠中，肾髓质间质给予高 NaCl 显著提高了肾组织尤其是内髓质和外髓质的 iNOS 活性，并大大增加了 UNO(x)排泄。因此，得出结论：在 NaCl 诱导的高血压情况下，诱导型 NOS 是血压的重要调节因子。
