Xue B J, Zhang X X, Shi G M, He R R
Department of Physiology and Hebei Medical University, Shijiazhuang 050017, China.
Sheng Li Xue Bao. 2000 Apr;52(2):159-62.
Effects of intracarotid injected capsaicin on the expression of Fos proto-oncogene in the brainstem nuclei involved in cardiovascular regulation were examined in 16 anesthetized rats with sinoaortic denervation. Only a few Fos-like protein immunoreactivity (FLI) neurons were found in the vehicle-control rats. Following intracarotid injection of capsaicin (10 micromol, 0.1 ml), the FLI neurons were markedly increased in nucleus paragigantocellularis lateralis (PGL), locus coeruleus (LC), area postrema (AP) and nucleus tractus solitarius (NTS), as compared with the results of vehicle-control rats, while the FLI neurons of raphe nuclei (RN) and periaqueductal gray (PAG) were not affected. The excitatory response to intracarotid injection of capsaicin was significantly inhibited by pretreatment with the vanilloid receptor (capsaicin receptor) antagonist ruthenium red (200 mmol, 0.1 ml). The results indicate that capsaicin may activate the neurons of the brainstem nuclei involved in cardiovascular regulation through vanilloid receptors, but does not exert any effects on PAG and RN.
在16只经窦神经和主动脉神经切断术的麻醉大鼠中，研究了颈内动脉注射辣椒素对参与心血管调节的脑干核中Fos原癌基因表达的影响。在注射溶剂的对照大鼠中仅发现少数Fos样蛋白免疫反应性（FLI）神经元。与注射溶剂的对照大鼠相比，颈内动脉注射辣椒素（10微摩尔，0.1毫升）后，外侧巨细胞旁核（PGL）、蓝斑（LC）、最后区（AP）和孤束核（NTS）中的FLI神经元明显增加，而中缝核（RN）和导水管周围灰质（PAG）的FLI神经元未受影响。用香草酸受体（辣椒素受体）拮抗剂钌红（200微摩尔，0.1毫升）预处理可显著抑制对颈内动脉注射辣椒素的兴奋反应。结果表明，辣椒素可能通过香草酸受体激活参与心血管调节的脑干核神经元，但对PAG和RN无任何影响。
