生长激素通过调节Bcl-2和半胱天冬酶-3来防止Fas诱导的淋巴细胞凋亡。
Growth hormone prevents Fas-induced apoptosis in lymphocytes through modulation of Bcl-2 and caspase-3.
作者信息
Mitsunaka H, Dobashi H, Sato M, Tanaka T, Kitanaka A, Yamaoka G, Tokuda M, Matoba K, Hiraishi T, Ishida T
机构信息
First Department of Internal Medicine, School of Medicine, Kagawa Medical University, Kita-gun, Kagawa, Japan.
出版信息
Neuroimmunomodulation. 2001;9(5):256-62. doi: 10.1159/000054288.
OBJECTIVE
Growth hormone (GH) has been reported to have a potent effect on the immune system. However, the detailed mechanism of the effect of GH on the immune system has not yet been clarified. This study was designed to investigate the nature of this mechanism.
METHODS
In the present study, we investigated the effects of GH on the susceptibility of both human CEM/C7 lymphocytes and human IM-9 lymphocytes to Fas-induced apoptosis.
RESULTS
Both cell lines expressed GH receptor mRNA. GH rescued Fas-induced suppression of [(3)H]-thymidine incorporation into each cell line. GH prevented Fas-induced apoptosis in each cell line without changing Fas antigen expression. We next investigated the mechanisms of the prevention of Fas-induced apoptosis, by focusing on intracellular molecules related to the apoptotic signal. Bcl-2 expression was increased by GH treatment in both CEM/C7 and IM-9 lymphocytes. GH also downregulated caspase-3 expression and inhibited activation of caspase-3 in both cell lines.
CONCLUSION
These findings suggest that GH regulates the human immune system through inhibition of Fas-induced apoptosis in activated T and B lymphocytes.
目的
据报道生长激素（GH）对免疫系统有强大作用。然而，GH对免疫系统作用的详细机制尚未阐明。本研究旨在探究该机制的本质。
方法
在本研究中，我们研究了GH对人CEM/C7淋巴细胞和人IM-9淋巴细胞对Fas诱导凋亡的易感性的影响。
结果
两种细胞系均表达GH受体mRNA。GH挽救了Fas诱导的对每个细胞系中[³H] - 胸腺嘧啶核苷掺入的抑制作用。GH在不改变Fas抗原表达的情况下，防止了每个细胞系中Fas诱导的凋亡。接下来，我们通过关注与凋亡信号相关的细胞内分子，研究了防止Fas诱导凋亡的机制。在CEM/C7和IM-9淋巴细胞中，GH处理均增加了Bcl-2表达。GH还下调了两种细胞系中caspase-3的表达并抑制了caspase-3的激活。
结论
这些发现表明，GH通过抑制活化的T和B淋巴细胞中Fas诱导的凋亡来调节人类免疫系统。
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