[双链RNA在真核基因沉默中的作用]

[Role of double-stranded RNA in eukaryotic gene silencing].

作者信息

Aravin A A, Klenov M S, Vagin V V, Rozovskiĭ Ia M, Gvozdev V A

机构信息

Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia.

出版信息

Mol Biol (Mosk). 2002 Mar-Apr;36(2):240-51.

PMID:11969085

Abstract

Data on RNA interference, that is, posttranscriptional gene silencing by homologous double-stranded (ds) RNA, are reviewed. Gene silencing caused by exogenous dsRNA in artificial systems and observed in transgenic organisms carrying additional gene copies is considered. Data are summarized on the mechanism that arose during evolution of the Drosophila melanogaster genome to suppress repetitive genes with the use of dsRNA and thereby to prevent male sterility. The role of dsRNA in inhibiting expression and transposition of mobile elements is discussed on the basis of authors own and published findings.

摘要

本文综述了RNA干扰的数据，即同源双链（ds）RNA引起的转录后基因沉默。文中考虑了在人工系统中外源dsRNA导致的基因沉默以及在携带额外基因拷贝的转基因生物中观察到的基因沉默现象。总结了在黑腹果蝇基因组进化过程中出现的一种机制的数据，该机制利用dsRNA抑制重复基因，从而防止雄性不育。基于作者自己的研究结果和已发表的研究发现，讨论了dsRNA在抑制移动元件表达和转座中的作用。