Maksimov G V, Nikandrov S L, Lazareva E S, Tychinskiĭ V P
Biological Department, Lomonosov Moscow State University, Vorob'evy Gory, Moscow, 119899 Russia.
Biofizika. 2002 Mar-Apr;47(2):345-51.
The method of dynamic phase microscopy was used to study the dynamics of changes in the structure of paranodal and nodal regions of a myelin nerve fiber of brown frog Rana temporaria at rest and under stimulation. Regular structural changes with frequencies of 5.3 and 10.8 Hz in the nodal region of the myelin nerve fiber were detected. A rhythmic excitation leads to additional changes in the structure of the nodal region with a new frequency of 5.6 Hz. It is likely that the regular changes in the nodal region of the myelin nerve induced by rhythmic excitation are due to slow changes in the axolemma (changes in the mode of lateral diffusion of membrane phospholipids), induced by developing trace changes in the membrane potential of the axolemma. The fact that these changes do not occur in the paranodal region of the fibre may indicate either the localization of regular structural changes in the axolemma or the difficulties that arise during the registration of the useful signal in the vicinity of myelin by this method.
运用动态相显微镜法，研究了处于静息状态和受刺激状态下的棕蛙（林蛙）有髓神经纤维结旁区和结区结构的动态变化。在有髓神经纤维的结区检测到频率为5.3赫兹和10.8赫兹的规则结构变化。节律性兴奋会导致结区结构出现频率为5.6赫兹的新的额外变化。有髓神经结区由节律性兴奋引起的规则变化，可能是由于轴膜的缓慢变化（膜磷脂横向扩散模式的变化）所致，而这种变化是由轴膜膜电位的微量变化发展而来的。纤维结旁区未出现这些变化这一事实，可能表明轴膜中规则结构变化的定位，或者是用这种方法在髓鞘附近记录有用信号时出现的困难。
