用于测量小鼠对随机共聚物（Glu60Phe40）反应的空斑形成细胞试验。
Plaque forming cell assay to measure responses in mice to the random copolymer (Glu60Phe40).
作者信息
Babu U M, Maurer P H, Merryman C F, Anderson J M
出版信息
Immunol Commun. 1979;8(5-6):531-8. doi: 10.3109/08820137909063251.
PMID:119705
Abstract
A plaque-forming cell (PFC) assay to measure the immune response of mice to the synthetic random copolymer of glutamic acid and phenylalanine (GPhe) is described here. GPhe can be coupled to sheep red blood cells (SRBC) using "aged" CrCl3. Both IgM and IgG plaques are detected in the murine GPhe response. Mice of H-2 haploytpes p and q are high responders, a and k are medium or low responders and b, d and s are nonresponders as detected by the PFC assay.
摘要
本文描述了一种用于测量小鼠对谷氨酸和苯丙氨酸合成无规共聚物（GPhe）免疫反应的空斑形成细胞（PFC）试验。GPhe可使用“老化的”三氯化铬与绵羊红细胞（SRBC）偶联。在小鼠对GPhe的反应中可检测到IgM和IgG空斑。通过PFC试验检测发现，H-2单倍型p和q的小鼠是高反应者，a和k是中或低反应者，而b、d和s是非反应者。
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