YgbQ是大肠杆菌和霍乱弧菌中的一种细胞分裂蛋白，它与FtsL以共依赖的方式定位于分裂位点。
YgbQ, a cell division protein in Escherichia coli and Vibrio cholerae, localizes in codependent fashion with FtsL to the division site.
作者信息
Buddelmeijer Nienke, Judson Nicholas, Boyd Dana, Mekalanos John J, Beckwith Jonathan
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):6316-21. doi: 10.1073/pnas.092128499. Epub 2002 Apr 23.
YgbQ is a cell division protein in Escherichia coli and Vibrio cholerae. In E. coli the ygbQ gene was discovered as a result of a computer search of the E. coli genome designed to find potential interacting partners for cell division protein FtsL. In V. cholerae, ygbQ was identified as an essential gene by using a transposon that fuses genes to an arabinose promoter. The role of YgbQ in cell division is supported by the following. Cells depleted of YgbQ in both organisms form long filaments, but DNA segregation is not affected. YgbQ localizes to the constriction site in wild-type E. coli cells. Localization of E. coli YgbQ to the constriction site depends on cell division proteins FtsQ and FtsL but not FtsW and FtsI, placing YgbQ in the sequential dependency order of proteins localizing to the division site. Localization of green fluorescent protein-FtsL also depends on YgbQ, indicating that FtsL and YgbQ colocalize to the division site in E. coli. Our results show colocalization of proteins to the bacterial midcell in E. coli and raise the possibility that these proteins interact in a coiled-coil structure.
YgbQ是大肠杆菌和霍乱弧菌中的一种细胞分裂蛋白。在大肠杆菌中，ygbQ基因是通过对大肠杆菌基因组进行计算机搜索而发现的，该搜索旨在寻找细胞分裂蛋白FtsL的潜在相互作用伙伴。在霍乱弧菌中，通过使用一种将基因与阿拉伯糖启动子融合的转座子，ygbQ被鉴定为一个必需基因。YgbQ在细胞分裂中的作用得到了以下支持。在这两种生物体中，缺乏YgbQ的细胞会形成长丝，但DNA分离不受影响。YgbQ定位于野生型大肠杆菌细胞的缢缩部位。大肠杆菌YgbQ定位于缢缩部位取决于细胞分裂蛋白FtsQ和FtsL，而不是FtsW和FtsI，这将YgbQ置于定位于分裂部位的蛋白质的顺序依赖性顺序中。绿色荧光蛋白-FtsL的定位也取决于YgbQ，表明FtsL和YgbQ在大肠杆菌中共同定位于分裂部位。我们的结果显示了蛋白质在大肠杆菌细菌中细胞中部的共定位，并增加了这些蛋白质以卷曲螺旋结构相互作用的可能性。
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