Vidal Benedicto De Campos, Joazeiro Paulo
Department of Cell Biology, Institute of Biology, State University of Campinas, P.O. Box 6109, 13083-970, SP, Campinas, Brazil.
Micron. 2002;33(5):507-9. doi: 10.1016/s0968-4328(01)00041-5.
The high positive linear dichroism (LD) exhibited by silver-impregnated collagen fibers are thought to be caused by an ordered binding of silver nanoparticles to the collagen fibers. A correlation between LD, established by image analysis, and the size of silver particles as measured by electron microscopy was investigated in the same sections of silver-impregnated tendon collagen fibers. Silver particles were found to be periodically ordered along the collagen fibers, with some preference for the 67 nm collagen period and collagen a bands. The silver particle diameter for a sample of n=279 was 18.51 nm on average, with 95% of the values ranging between 17.90 and 19.14 nm. The finding that interference colors are correlated to abnormal dispersion of birefringence characteristics supports the concept that collagen fibers induce an ordered binding of silver distributed along the fibers.
银浸渍的胶原纤维呈现出的高正线性二色性（LD）被认为是由银纳米颗粒与胶原纤维的有序结合引起的。在银浸渍的肌腱胶原纤维的相同切片中，研究了通过图像分析确定的LD与通过电子显微镜测量的银颗粒大小之间的相关性。发现银颗粒沿着胶原纤维呈周期性排列，对67nm的胶原周期和胶原α带具有一定偏好。n = 279的样本中银颗粒直径平均为18.51nm，95%的值在17.90至19.14nm之间。干涉色与双折射特性的异常色散相关这一发现支持了胶原纤维诱导沿纤维分布的银有序结合的概念。
