Iwatsuki Hirohiko, Ogawa Chizuru, Suda Masumi
Department of Anatomy, Kawasaki Medical School, Matsushima 577, Kurashiki 701-0192, Japan,
Histochem Cell Biol. 2002 Apr;117(4):363-70. doi: 10.1007/s00418-002-0389-z. Epub 2002 Mar 1.
In rabbit intestinal epithelium, vimentin intermediate filaments are selectively expressed in the M cells of follicle-associated epithelium (FAE). To find intestinal epithelial cells belonging to the M cell lineage, vimentin was detected immunohistochemically in the rabbit small and large intestines. Vimentin-positive columnar cells were scattered throughout the villus epithelium of the small intestine. In their cytoplasm, vimentin was located from the perinuclear region to the cell membrane touching intraepithelial lymphocytes. These cells had microvilli shorter than those of absorptive cells, and the alkaline phosphatase activity of the microvilli was markedly weaker than that of absorptive cell microvilli. Glycoconjugates on the surface of the microvilli were alcian blue positive and periodic acid-Schiff negative. The morphological and histochemical features of these vimentin-positive villus epithelial cells differed from those of adjacent absorptive cells and closely resembled those of the M cells in FAE covering Peyer's patches and solitary lymphatic nodules. These results suggest that the vimentin-positive cells in the villus epithelium belong to the M cell lineage.
在兔肠上皮中，波形蛋白中间丝在滤泡相关上皮（FAE）的M细胞中选择性表达。为了找到属于M细胞谱系的肠上皮细胞，采用免疫组织化学方法在兔的小肠和大肠中检测波形蛋白。波形蛋白阳性柱状细胞散在于小肠绒毛上皮中。在其细胞质中，波形蛋白位于核周区域至接触上皮内淋巴细胞的细胞膜。这些细胞的微绒毛比吸收细胞的短，且微绒毛的碱性磷酸酶活性明显弱于吸收细胞微绒毛。微绒毛表面的糖缀合物阿尔辛蓝阳性、过碘酸希夫阴性。这些波形蛋白阳性绒毛上皮细胞的形态和组织化学特征与相邻的吸收细胞不同，与覆盖派伊尔结和孤立淋巴小结的FAE中的M细胞非常相似。这些结果表明，绒毛上皮中的波形蛋白阳性细胞属于M细胞谱系。
