Tanaka Yukichi, Carney J Aidan, Ijiri Rieko, Kato Keisuke, Miyake Tetsumi, Nakatani Yukio, Misugi Kazuaki
Division of Pathology, Kanagawa Children's Medical Center, Yokohama, Japan.
Hum Pathol. 2002 Mar;33(3):285-9. doi: 10.1053/hupa.2002.32224.
This study concerns the immunohistochemical localization of S-100 alpha, S-100 beta, and whole brain S-100 (wbS-100) in testicular large-cell calcifying Sertoli cell tumor (LCCSCT). We examined 8 LCCSCTs (7 benign and 1 malignant), 6 Sertoli cell tumors not otherwise specified (SCTs-NOS), 6 Leydig cell tumors (LCTs), 5 ovarian Sertoli-Leydig cell tumors (SLCTs), and 7 gonadoblastomas (GBLs). The 8 LCCSCTs showed immunoreactivity for S-100 alpha, S-100 beta, and wbS-100. Five of the 6 LCTs and the Leydig cell components in the ovarian SLCTs stained positively for S-100 alpha and wbS-100 but were negative for S-100 beta. SCTs-NOS and the Sertoli cell components in the SLCTs occasionally showed focal and weak/moderate positivity for S-100 alpha, S-100 beta, and wbS-100. Sex cord cells of the GBLs were positive for S-100 beta and wbS-100 and negative for S-100 alpha. Germ cell elements of the GBLs were negative for S-100 alpha, S-100 beta, and wbS-100. In nonneoplastic testicular parenchyma adjacent to the above-mentioned tumors, there was S-100 alpha reactivity in Leydig cells, rete testis, and a few Sertoli cells. S-100 beta reactivity was seen in a few Sertoli cells, Schwann cells, and some endothelial cells. WbS-100 reactivity was present in Leydig cells, a few Sertoli cells, rete testis, Schwann cells, and some endothelial cells. The results indicate that S-100 alpha and S-100 beta can potentially be used as immunohistochemical markers for LCCSCT, especially when differentiating it from LCT, which may mimic LCCSCT on routine histopathology. Although the biological significance of both S-100 subunits expression in LCCSCT remains unknown, these notable calcium-binding proteins may be associated with the characteristic calcification in LCCSCT through regulation of calcium levels in the tumor cells.
本研究关注S-100α、S-100β和全脑S-100（wbS-100）在睾丸大细胞钙化性支持细胞瘤（LCCSCT）中的免疫组织化学定位。我们检测了8例LCCSCT（7例良性和1例恶性）、6例未另行指定的支持细胞瘤（SCTs-NOS）、6例间质细胞瘤（LCTs）、5例卵巢支持-间质细胞瘤（SLCTs）和7例性腺母细胞瘤（GBLs）。8例LCCSCT对S-100α、S-100β和wbS-100均呈免疫反应性。6例LCT中的5例以及卵巢SLCTs中的间质细胞成分对S-100α和wbS-100呈阳性染色，但对S-100β呈阴性。SCTs-NOS以及SLCTs中的支持细胞成分偶尔对S-100α、S-100β和wbS-100呈局灶性弱阳性/中度阳性。GBLs的性索细胞对S-100β和wbS-100呈阳性，对S-100α呈阴性。GBLs的生殖细胞成分对S-100α、S-100β和wbS-100呈阴性。在上述肿瘤相邻的非肿瘤性睾丸实质中，间质细胞、睾丸网和少数支持细胞中有S-100α反应性。少数支持细胞、施万细胞和一些内皮细胞中有S-100β反应性。间质细胞、少数支持细胞、睾丸网、施万细胞和一些内皮细胞中有wbS-100反应性。结果表明，S-100α和S-100β有可能用作LCCSCT的免疫组织化学标志物，尤其是在将其与LCT鉴别时，LCT在常规组织病理学上可能与LCCSCT相似。尽管LCCSCT中S-100两个亚基表达的生物学意义尚不清楚，但这些显著的钙结合蛋白可能通过调节肿瘤细胞中的钙水平与LCCSCT的特征性钙化有关。
