Dorfman David M, Shahsafaei Aliakbar
Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Hum Pathol. 2002 Mar;33(3):330-4. doi: 10.1053/hupa.2002.32215.
CD69, a marker of early T cell activation, is associated with Th1 T cell differentiation. Previously we found that peripheral T cell lymphomas could be subdivided based on the expression of markers of Th1 versus Th2 differentiation, including CXCR3, CD134/OX40, CCR4, and CD30. Here we report immunohistochemical staining for CD69 in frozen and paraffin sections of peripheral T cell lymphomas that exhibit immunoreactivity for markers of Th1 or Th2 differentiation. CD69 expression correlated with immunoreactivity for other Th1 differentiation markers in 18 of 19 frozen specimens of peripheral T cell lymphomas (P = 0.0005). In 10 of these cases in which paraffin-embedded tissue was available for study, CD69 immunohistochemical staining of paraffin sections correlated with frozen section expression. CD69 immunostaining was performed on paraffin sections from 53 additional cases of peripheral T cell lymphoma and correlated with immunoreactivity for other Th1 differentiation markers (P < 0.0001) and was associated with specific subtypes of peripheral T cell lymphoma, including angioimmunoblastic lymphoma, Lennert's lymphoma, and mycosis fungoides/Sezary syndrome, previously noted to express Th1 differentiation-associated markers. Anaplastic large cell lymphoma, both systemic and cutaneous, which typically exhibits immunoreactivity for markers of Th2 expression, was negative for CD69 immunostaining in 22 of 24 cases. CD69 immunostaining results support previous findings that a subset of T cell lymphomas exhibits immunophenotypic features of either Th1 or Th2 T cell differentiation. In addition, CD69 is a useful immunohistochemical marker for specific T cell lymphomas in frozen and paraffin-embedded tissue.
CD69是早期T细胞活化的标志物，与Th1 T细胞分化相关。此前我们发现，外周T细胞淋巴瘤可根据Th1与Th2分化标志物的表达进行细分，这些标志物包括CXCR3、CD134/OX40、CCR4和CD30。在此我们报告了对外周T细胞淋巴瘤冰冻切片和石蜡切片进行CD69免疫组化染色的结果，这些外周T细胞淋巴瘤对Th1或Th2分化标志物呈免疫反应性。在19例外周T细胞淋巴瘤冰冻标本中的18例中，CD69表达与其他Th1分化标志物的免疫反应性相关（P = 0.0005）。在其中10例有石蜡包埋组织可供研究的病例中，石蜡切片的CD69免疫组化染色与冰冻切片表达相关。对另外53例外周T细胞淋巴瘤的石蜡切片进行了CD69免疫染色，并与其他Th1分化标志物的免疫反应性相关（P < 0.0001），且与外周T细胞淋巴瘤的特定亚型相关，包括血管免疫母细胞性淋巴瘤、 Lennert淋巴瘤以及蕈样肉芽肿/赛塞里综合征，这些亚型之前已被指出表达Th1分化相关标志物。24例系统性和皮肤性间变性大细胞淋巴瘤中，有22例对Th2表达标志物呈免疫反应性，其CD69免疫染色为阴性。CD69免疫染色结果支持了之前的发现，即一部分T细胞淋巴瘤表现出Th1或Th2 T细胞分化的免疫表型特征。此外，CD69是冰冻和石蜡包埋组织中特定T细胞淋巴瘤的一种有用的免疫组化标志物。
