Khattak Shahryar, Im Hogune, Park Taeju, Ahnn Joohong, Spoerel Nikolaus A
Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, Korea.
Cell Biochem Funct. 2002 Jun;20(2):119-27. doi: 10.1002/cbf.960.
ELL (Eleven-nineteen Lysine rich Leukemia) is known to be an elongation factor resembling elongin for RNA polymerase II transcription. A homologue of human ELL (hELL) was identified in Drosophila melanogaster (dELL) and several cDNA clones were isolated from the embryonic cDNA library. We showed that dELL is expressed mainly in the ovaries and early embryonic stages by developmental Northern blot. dELL encodes a protein of 912 amino acids which is substantially longer than the hELL (612 aa). Immunostaining revealed that dELL was localized to nuclei in early embryos and to nuclei of nurse cells and follicle cells in the ovary suggesting its important role in early development of drosophila. To elucidate the function of this gene in drosophila, P-element mobilization was performed by utilizing a P-element inserted upstream of dELL. Southern analysis showed that isolated mutants are internal P-element deletions. These P-element deletions can now be used to isolate dELL mutations by EMS mutagenesis.
ELL（富含11-19位赖氨酸的白血病蛋白）是一种已知的类似于RNA聚合酶II转录延伸因子elongin的延伸因子。在黑腹果蝇（dELL）中鉴定出了人类ELL（hELL）的同源物，并从胚胎cDNA文库中分离出了几个cDNA克隆。通过发育Northern印迹法，我们发现dELL主要在卵巢和胚胎早期阶段表达。dELL编码一种由912个氨基酸组成的蛋白质，其长度明显长于hELL（612个氨基酸）。免疫染色显示，dELL在早期胚胎中定位于细胞核，在卵巢中定位于滋养细胞和卵泡细胞的细胞核，这表明它在果蝇早期发育中具有重要作用。为了阐明该基因在果蝇中的功能，利用插入dELL上游的P因子进行P因子动员。Southern分析表明，分离出的突变体是内部P因子缺失。这些P因子缺失现在可用于通过EMS诱变分离dELL突变体。
