Fuchsjäger-Mayrl Gabriele, Malec Magdalena, Polska Elzbieta, Jilma Bernd, Wolzt Michael, Schmetterer Leopold
Department of Clinical Pharmacology, Vienna University, Währinger Gürtel 18-20, A-1090 Vienna, Austria.
Invest Ophthalmol Vis Sci. 2002 May;43(5):1520-4.
The blue-field entoptic technique was introduced more than 20 years ago to quantify perimacular white blood cell flux. However, a final confirmation that the perceived corpuscles represent leukocytes is still unavailable.
The study design was randomized, placebo-controlled, and double masked with two parallel groups. Fifteen healthy male subjects received a single dose of granulocyte colony stimulating factor (G-CSF, 300 microg) and 15 other subjects received placebo. The following parameters were assessed at baseline and at 12 minutes and 8 hours after administration: retinal white blood cell flux, with the blue-field entoptic technique; retinal blood velocities, with bidirectional laser Doppler velocimetry; retinal venous diameter determined with a retinal vessel analyzer; and blood pressure and pulse rate determined by automated oscillometry and pulse oxymetry, respectively.
After 12 minutes, G-CSF reduced total leukocyte count from 5.5 +/- 1.4 10(9)/L at baseline to 1.9 +/- 0.4 10(9)/L. This was paralleled by a 35% +/- 11% decrease in retinal white blood cell density. After 8 hours G-CSF increased total leukocyte counts to 20.0 +/- 4.4 10(9)/L. Again, this increase in circulating leukocytes was reflected by an increase in retinal white blood cell density (110% +/- 48%). All effects were significant at P < 0.001. By contrast, none of the other hemodynamic parameters was changed by administration of G-CSF.
The results clearly indicate that the blue-field entoptic technique assesses leukocyte movement in the perimacular capillaries of the retina. Moreover, white blood cell density appears to adequately reflect the number of circulating leukocytes within the retinal microvasculature. Hence, an increase in retinal white blood cell density does not necessarily reflect retinal vasodilatation.
20多年前引入蓝视场眼内现象技术来量化黄斑周围白细胞流量。然而，对于所观察到的微粒代表白细胞这一点仍未得到最终证实。
研究设计为随机、安慰剂对照、双盲且有两个平行组。15名健康男性受试者接受单剂量粒细胞集落刺激因子（G-CSF，300微克），另外15名受试者接受安慰剂。在基线以及给药后12分钟和8小时评估以下参数：采用蓝视场眼内现象技术测量视网膜白细胞流量；采用双向激光多普勒测速法测量视网膜血流速度；用视网膜血管分析仪测定视网膜静脉直径；分别通过自动示波法和脉搏血氧测定法测定血压和脉搏率。
12分钟后，G-CSF使总白细胞计数从基线时的5.5±1.4×10⁹/L降至1.9±0.4×10⁹/L。与此同时，视网膜白细胞密度下降了35%±11%。8小时后，G-CSF使总白细胞计数增至20.0±4.4×10⁹/L。同样，循环白细胞的这种增加反映在视网膜白细胞密度增加（110%±48%）。所有效应在P<0.001时均具有显著性。相比之下，给予G-CSF后其他血流动力学参数均未改变。
结果清楚地表明，蓝视场眼内现象技术可评估视网膜黄斑周围毛细血管中的白细胞移动。此外，白细胞密度似乎能充分反映视网膜微血管系统内循环白细胞的数量。因此，视网膜白细胞密度的增加不一定反映视网膜血管扩张。
