Ohtsuki Iwao, Shiraishi Fumie
Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
J Biochem. 2002 May;131(5):739-43. doi: 10.1093/oxfordjournals.jbchem.a003159.
We investigated the distribution of troponin C.I and troponin I along tropomyosin-actin filaments by immunoelectron microscopy and found that anti-troponin I antibody formed transverse striations at 38 nm intervals along the bundle of filaments of both troponin C.I-tropomyosin-actin and troponin I-tropomyosin-actin. Since the length of 38 nm corresponds to the repeating period of filamentous tropomyosin along actin double strands, the present study indicates that troponin I is located at a specific region of each tropomyosin, suggesting that a specific interaction between troponin I and tropomyosin is involved in determining the periodic distribution of troponin I along tropomyosin-actin filaments.
我们通过免疫电子显微镜研究了肌钙蛋白C.I和肌钙蛋白I沿原肌球蛋白-肌动蛋白丝的分布，发现抗肌钙蛋白I抗体在肌钙蛋白C.I-原肌球蛋白-肌动蛋白和肌钙蛋白I-原肌球蛋白-肌动蛋白的丝束上以38nm的间隔形成横向条纹。由于38nm的长度对应于原肌球蛋白沿肌动蛋白双链的重复周期，本研究表明肌钙蛋白I位于每个原肌球蛋白的特定区域，这表明肌钙蛋白I与原肌球蛋白之间的特定相互作用参与了决定肌钙蛋白I沿原肌球蛋白-肌动蛋白丝的周期性分布。
