熊去氧胆酸促进胆管梗阻大鼠部分肝切除术后的肝再生

[Ursodeoxycholic acid promotes liver regeneration after partial hepatectomy in bile duct obstructive rats].

作者信息

Xu Mingqing, Han Benli, Xue Lan, Gong Jianping

机构信息

Department of Hepatobiliary Surgery, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2002 Apr;10(2):103-5.

PMID:11983124

Abstract

OBJECTIVE

To investigate the effect of ursodeoxycholic acid (UDCA) on liver regeneration after 70% partial hepatectomy (PH) in bile duct obstructive (BDO) rats.

METHODS

Wistar rats were randomly divided into N-PH group in which normal rats were operated with 70% PH, BDO-PH group in which 70% PH were operated after two week's BDO, and BDO-PH UDCA or sterile saline treatment group in which UDCA (15mg kg(-1) d(-1)) or saline was administrated during BDO and after 70% PH. The hepatic pathological changes were observed. BrdU labeling of hepatocytes, the mRNA expression of intrahepatic hepatocyte growth factor (HGF) and its receptor (Met gene) after 70% PH were measured by immunohistochemical analysis and RT-PCR, respectively.

RESULTS

Improvements of hepatic function and pathological changes were induced by UDCA administration after BDO. The expression of hepatic HGF/Met mRNA after 70% PH in BDO-PH UDCA treatment group rats was significantly increased compared with N-PH group rats (P<0.05), BrdU peak labelling of hepatocytes (59.39% +/- 10.82%) in BDO-PH UDCA treatment group rats was significantly higher than that (36.22% +/- 8.37%) in BDO-PH group rats (t=4.149, P<0.01) and without significance compared with N-PH group rats (68.64% +/- 11.26%, t=1.451, P >0.05).

CONCLUSIONS

UDCA promotes liver regeneration after 70% PH in BDO rats by remission of hepatic pathological changes and elevating hepatic mRNA expression of HGF and Met.

摘要

目的

研究熊去氧胆酸（UDCA）对胆管梗阻（BDO）大鼠70%肝部分切除术（PH）后肝再生的影响。

方法

将Wistar大鼠随机分为正常大鼠行70% PH的N-PH组、胆管梗阻2周后行70% PH的BDO-PH组，以及在胆管梗阻期间和70% PH后给予UDCA（15mg kg⁻¹ d⁻¹）或生理盐水的BDO-PH UDCA或无菌生理盐水治疗组。观察肝脏病理变化。分别通过免疫组化分析和RT-PCR检测70% PH后肝细胞的BrdU标记、肝内肝细胞生长因子（HGF）及其受体（Met基因）的mRNA表达。

结果

BDO后给予UDCA可改善肝功能和病理变化。BDO-PH UDCA治疗组大鼠70% PH后肝HGF/Met mRNA表达较N-PH组大鼠显著增加（P<0.05），BDO-PH UDCA治疗组大鼠肝细胞的BrdU峰值标记（59.39%±10.82%）显著高于BDO-PH组大鼠（36.22%±8.37%，t=4.149，P<0.01），与N-PH组大鼠（68.64%±11.26%，t=1.451，P>0.05）相比无显著差异。