Xu Mingqing, Han Benli, Xue Lan, Gong Jianping
Department of Hepatobiliary Surgery, Southwestern Hospital, Third Military Medical University, Chongqing 400038, China.
Zhonghua Gan Zang Bing Za Zhi. 2002 Apr;10(2):103-5.
To investigate the effect of ursodeoxycholic acid (UDCA) on liver regeneration after 70% partial hepatectomy (PH) in bile duct obstructive (BDO) rats.
Wistar rats were randomly divided into N-PH group in which normal rats were operated with 70% PH, BDO-PH group in which 70% PH were operated after two week's BDO, and BDO-PH UDCA or sterile saline treatment group in which UDCA (15mg kg(-1) d(-1)) or saline was administrated during BDO and after 70% PH. The hepatic pathological changes were observed. BrdU labeling of hepatocytes, the mRNA expression of intrahepatic hepatocyte growth factor (HGF) and its receptor (Met gene) after 70% PH were measured by immunohistochemical analysis and RT-PCR, respectively.
Improvements of hepatic function and pathological changes were induced by UDCA administration after BDO. The expression of hepatic HGF/Met mRNA after 70% PH in BDO-PH UDCA treatment group rats was significantly increased compared with N-PH group rats (P<0.05), BrdU peak labelling of hepatocytes (59.39% +/- 10.82%) in BDO-PH UDCA treatment group rats was significantly higher than that (36.22% +/- 8.37%) in BDO-PH group rats (t=4.149, P<0.01) and without significance compared with N-PH group rats (68.64% +/- 11.26%, t=1.451, P >0.05).
UDCA promotes liver regeneration after 70% PH in BDO rats by remission of hepatic pathological changes and elevating hepatic mRNA expression of HGF and Met.
研究熊去氧胆酸（UDCA）对胆管梗阻（BDO）大鼠70%肝部分切除术（PH）后肝再生的影响。
将Wistar大鼠随机分为正常大鼠行70% PH的N-PH组、胆管梗阻2周后行70% PH的BDO-PH组，以及在胆管梗阻期间和70% PH后给予UDCA（15mg kg⁻¹ d⁻¹）或生理盐水的BDO-PH UDCA或无菌生理盐水治疗组。观察肝脏病理变化。分别通过免疫组化分析和RT-PCR检测70% PH后肝细胞的BrdU标记、肝内肝细胞生长因子（HGF）及其受体（Met基因）的mRNA表达。
BDO后给予UDCA可改善肝功能和病理变化。BDO-PH UDCA治疗组大鼠70% PH后肝HGF/Met mRNA表达较N-PH组大鼠显著增加（P<0.05），BDO-PH UDCA治疗组大鼠肝细胞的BrdU峰值标记（59.39%±10.82%）显著高于BDO-PH组大鼠（36.22%±8.37%，t=4.149，P<0.01），与N-PH组大鼠（68.64%±11.26%，t=1.451，P>0.05）相比无显著差异。
UDCA通过减轻肝脏病理变化、提高肝脏HGF和Met的mRNA表达，促进BDO大鼠70% PH后的肝再生。
