Wang Shaoyang, Lin Yumei, Ma Weimin, Zhang Baohua, Qi Shaoran, Lan Fenghua
Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350003, China.
Zhonghua Gan Zang Bing Za Zhi. 2002 Apr;10(2):116-9.
To investigate the effect of IL-12 on IFN-gamma and IL-10 production of peripheral blood mononuclear cells (PBMC) in chronic hepatitis B virus infection patients during IFN-alpha treatment.
Before and after IFN-alpha treatment of 3 months and 6 months, PBMC of 20 patients with chronic hepatitis B virus infection were collected and cultured in vitro in the culture fluid containing PHA (100 microg/ml), HBcAg (1 microg/ml), or HBeAg (1 microg/ml) for 48 h under the condition of the presence or absence of IL-12 (10 ng/ml). Then the levels of IFN-gamma and IL-10 were determined by ELISA.
There were 12 responders and 8 nonresponders to IFN-alpha treatment. In the responders, the enhancing effect of IL-12 on IFN-gamma production was significantly greater after IFN-alpha treatment than before the treatment. The production of IL-10 was suppressed in the presence of IL-12 after 3 months and 6 months of IFN-alpha treatment. In the same treatment time, the level of IFN-gamma in the presence of IL-12 was significantly higher than that in the absence of IL-12. To the nonresponders, the enhancing effect of IL-12 on IFN-gamma production was also significantly increased after IFN-alpha treatment. Moreover, in the same treatment time, the level of IFN-gamma in the presence of IL-12 was significantly higher than that in the absence of IL-12.
The enhancing effect of IL-12 on IFN-gamma production of PBMC in patients with chronic hepatitis B virus infection is increased during IFN-alpha treatment. IFN-alpha and IL-12 may enhance the efficacy for the treatment of chronic hepatitis B virus infection.
探讨白细胞介素-12（IL-12）对慢性乙型肝炎病毒感染患者在α干扰素治疗期间外周血单个核细胞（PBMC）中γ干扰素（IFN-γ）和白细胞介素-10（IL-10）产生的影响。
在20例慢性乙型肝炎病毒感染患者接受α干扰素治疗3个月和6个月前后，采集其PBMC，在含或不含IL-12（10 ng/ml）的情况下，于含有PHA（100 μg/ml）、乙肝核心抗原（HBcAg，1 μg/ml）或乙肝e抗原（HBeAg，1 μg/ml）的培养液中体外培养48小时。然后采用酶联免疫吸附测定法（ELISA）检测IFN-γ和IL-10水平。
α干扰素治疗有效者12例，无效者8例。在有效者中，IL-12对IFN-γ产生的增强作用在α干扰素治疗后比治疗前显著增强。α干扰素治疗3个月和6个月后，在有IL-12存在时IL-10的产生受到抑制。在相同治疗时间，有IL-12存在时IFN-γ水平显著高于无IL-12时。对无效者而言，IL-12对IFN-γ产生的增强作用在α干扰素治疗后也显著增强。此外，在相同治疗时间，有IL-12存在时IFN-γ水平显著高于无IL-12时。
在α干扰素治疗期间，IL-12对慢性乙型肝炎病毒感染患者PBMC中IFN-γ产生的增强作用增强。α干扰素和IL-12可能增强慢性乙型肝炎病毒感染的治疗效果。
