糖尿病和急性高血压对大鼠血浆一氧化氮和内皮素浓度的影响。

Effects of diabetes mellitus and acute hypertension on plasma nitric oxide and endothelin concentrations in rats.

作者信息

Vural Pervin, Cevik Aydin, Curgunlu Asli, Canbaz Mukaddes

机构信息

Department of Biochemistry, Istanbul Medical Faculty, Capa 34390, Istanbul, Turkey.

出版信息

Clin Chim Acta. 2002 Jun;320(1-2):43-7. doi: 10.1016/s0009-8981(02)00024-4.

DOI:10.1016/s0009-8981(02)00024-4

PMID:11983199

Abstract

AIM

To examine the plasma nitrate/nitrite (NOx-two end products of the nitric oxide metabolism) and endothelin (ET) concentrations, and response to acute adrenaline induced hypertension in diabetic rats.

MATERIALS AND METHODS

Four groups of 4-month-old rats were used: control rats (C, n=10) rats received adrenaline (A, 40 microg/kg i.v., n=10), rats received streptozotocin (S, 50 mg/kg i.v., n=8), and rats received STZ and adrenaline (SA, n=9). The experiments were performed 4 weeks after the STZ administration. Plasma NOx, ET, glucose, and mean arterial blood pressure (MAP) were measured.

RESULTS

Plasma ET concentrations were significantly increased in diabetic rats (S and SA) in comparison with the controls and adrenaline-only administered rats. NOx concentrations in diabetic groups (S and SA) were significantly decreased in comparison with the controls. Acute adrenaline induced hypertension in diabetes leads to a significant decrease of NOx concentrations in comparison with the controls, adrenaline-only administered and STZ-only administered rats. There was no difference between the MAP in diabetic and control rats. Adrenaline injection caused a significant increase of MAP in A and SA groups. Plasma glucose concentrations in diabetic rats (S and SA) were significantly increased in comparison with the nondiabetic groups (C and A). There was a weak but significant correlation between the NOx and ET concentrations in the controls, which probably reveal the balance between these vasoactive factors. In A, S, and SA groups, no significant correlation between the NOx/ET was found.

CONCLUSION

An impairment of the NOx and ET formation could be involved in the pathogenesis of diabetes mellitus and especially acute hypertension and diabetes. A lack of correlation between the NOx and ET probably indicated that in diabetes and acute hypertension, a primary mechanism of compensatory nitric oxide might be lost.

摘要

目的

检测糖尿病大鼠血浆中硝酸盐/亚硝酸盐（NOx，一氧化氮代谢的两种终产物）和内皮素（ET）的浓度，以及对急性肾上腺素诱导的高血压的反应。

材料与方法

使用四组4月龄大鼠：对照大鼠（C组，n = 10）、接受肾上腺素的大鼠（A组，静脉注射40μg/kg，n = 10）、接受链脲佐菌素的大鼠（S组，静脉注射50mg/kg，n = 8）以及接受链脲佐菌素和肾上腺素的大鼠（SA组，n = 9）。链脲佐菌素给药4周后进行实验。测量血浆NOx、ET、葡萄糖和平均动脉血压（MAP）。

结果

与对照组和仅给予肾上腺素的大鼠相比，糖尿病大鼠（S组和SA组）的血浆ET浓度显著升高。与对照组相比，糖尿病组（S组和SA组）的NOx浓度显著降低。糖尿病状态下急性肾上腺素诱导的高血压导致与对照组、仅给予肾上腺素组和仅给予链脲佐菌素组相比，NOx浓度显著降低。糖尿病大鼠和对照大鼠的MAP无差异。肾上腺素注射导致A组和SA组的MAP显著升高。与非糖尿病组（C组和A组）相比，糖尿病大鼠（S组和SA组）的血浆葡萄糖浓度显著升高。对照组中NOx和ET浓度之间存在微弱但显著的相关性，这可能揭示了这些血管活性因子之间的平衡。在A组、S组和SA组中，未发现NOx/ET之间存在显著相关性。