Tang Zhongshu, Yu Yi, Guo Hong, Zhou Jiawei
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, 200031, PR China.
Neurosci Lett. 2002 May 10;324(1):13-6. doi: 10.1016/s0304-3940(02)00153-2.
Differentiation of neural stem cells into tyrosine hydroxylase (TH)-expressing cells was studied by cell transplantation into the various brain regions of rats that had received 6-hydroxydopamine lesion of the nigrostriatal pathway. Approximately 13.6-16.1% of survived precursor cells acquired neuronal-like features by expressing the neuronal marker doublecortin. Similarly, 20.7-25.7% of survived precursor cells differentiated into astrocytes following transplantation. Immunohistochemical analysis revealed that a fraction of the grafted precursor cells in the anterior part of the medial forebrain bundle (MFB), but not in the striatum and the substantia nigra of the lesioned side, showed strong TH immunoreactivity. This suggests that MFB is more permissive for induction of TH to the striatal precursor cells in vivo. The results further exemplify the potential of neural stem cells and the property of site-specific differentiation when the cells were transplanted to the dopaminergic system of the adult brain.
通过将细胞移植到接受了黑质纹状体通路6-羟基多巴胺损伤的大鼠的不同脑区，研究神经干细胞向表达酪氨酸羟化酶（TH）的细胞的分化。大约13.6 - 16.1%存活的前体细胞通过表达神经元标志物双皮质素获得了神经元样特征。同样，移植后20.7 - 25.7%存活的前体细胞分化为星形胶质细胞。免疫组织化学分析显示，在内侧前脑束（MFB）前部的一部分移植前体细胞呈现强烈的TH免疫反应性，而在损伤侧的纹状体和黑质中则没有。这表明MFB在体内对纹状体前体细胞诱导TH表达更具适应性。这些结果进一步例证了神经干细胞的潜力以及当细胞移植到成年大脑的多巴胺能系统时的位点特异性分化特性。
