Perrone Paola, Hewage Chandralal M, Thomson Adam R, Bailey Kevin, Sadler Ian H, Fry Stephen C
The Edinburgh Cell Wall Group, Institute of Cell and Molecular Biology, The University of Edinburgh, UK.
Phytochemistry. 2002 May;60(1):67-77. doi: 10.1016/s0031-9422(02)00039-0.
Driselase-digestion of cell walls from suspension-cultures of spinach (Spinacia oleracea L.), followed by anion-exchange chromatography, gel-permeation chromatography, preparative paper chromatography and preparative paper electrophoresis, yielded ten uronic acid-containing products in addition to free galacturonic acid (GalA). These included 4-O-methylglucuronic acid, alpha-L-rhamnopyranosyl-(1-->4)-D-glucuronic acid and several oligosaccharides containing GalA residues. The structures were unambiguously determined by a combination of 1- and 2-dimensional NMR spectroscopic techniques. Five of the six homogalacturonan-derived oligosaccharides purified contained 3-O-acetyl-GalA residues; however, methyl-esterified GalA residues occurred adjacent to both 2-O-acetyl-GalA and 3-O-acetyl-GalA residues. An acetylated, rhamnogalacturonan-I-derived oligosaccharide that was purified also contained 3-O-acetyl-GalA residues. Taken together with published data, our findings indicate considerable diversity in the patterns of pectin esterification. The implications for the action of pectin esterases are discussed.
用Driselase酶消化菠菜（Spinacia oleracea L.）悬浮培养细胞的细胞壁，然后依次进行阴离子交换色谱、凝胶渗透色谱、制备型纸色谱和制备型纸电泳，除了游离半乳糖醛酸（GalA）外，还得到了十种含糖醛酸的产物。这些产物包括4-O-甲基葡萄糖醛酸、α-L-鼠李吡喃糖基-(1→4)-D-葡萄糖醛酸以及几种含有GalA残基的寡糖。通过一维和二维核磁共振光谱技术相结合，明确确定了这些结构。纯化得到的六种源自同型半乳糖醛酸聚糖的寡糖中，有五种含有3-O-乙酰基-GalA残基；然而，甲酯化的GalA残基与2-O-乙酰基-GalA和3-O-乙酰基-GalA残基相邻。纯化得到的一种乙酰化的、源自鼠李半乳糖醛酸聚糖-I的寡糖也含有3-O-乙酰基-GalA残基。结合已发表的数据，我们的研究结果表明果胶酯化模式具有相当大的多样性。文中还讨论了其对果胶酯酶作用的影响。
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